Immunohistochemical localization of phospholipase Dl in rat central nervous system

Mun Yong Lee, Yang Hyeok Jo, Myung Hoon Chun, Jin Woong Chung, Myung Suk Kim, Do Sik Min

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Phospholipase D (PLD) is one of the intracellular signal transduction enzymes and plays an important role in a variety of cellular functions. We investigated the distribution of PLD isozyme, PLD1 in the rat brain and spinal cord using an immunological approach. Western blot analysis showed the presence of PLD1 protein in all tissues studied, with significantly higher levels in the brainstem and spinal cord, which was correlated with the results obtained from PLD activity assay. Prominent and specific signals of PLD1 were observed in many functionally diverse brain areas, including the olfactory bulb, medial septum-diagonal band complex, cerebral cortex, brainstem, cerebellum, and spinal cord. In the brainstem, the red nucleus, substantia nigra, interpeduncular nucleus, cranial motor nuclei (trigeminal motor, abducent, facial, and hypoglossal), sensory cranial nerve nuclei (spinal trigeminal, vestibular, and cochlear), as well as nuclei of the reticular formation, all showed intense immunoreactivity. Purkinje cells and deep cerebellar nuclei of the cerebellum were also labeled intensely. However, no significant labeling was found in the thalamus, epithalamus, and basal ganglia. Although many of the PLD1 immunoreactive cells were neurons, PLD1 was also expressed in glial cells such as presumed astrocytes and tanycytes. These findings suggest that PLD1 may play an important role in the central nervous system of the adult rat.

Original languageEnglish
Pages (from-to)52-59
Number of pages8
JournalBrain Research
Volume864
Issue number1
DOIs
Publication statusPublished - 2000 May 5

Fingerprint

Phospholipase D
Phospholipases
Brain Stem
Spinal Cord
Central Nervous System
Cerebellum
Epithalamus
Spinal Trigeminal Nucleus
Red Nucleus
Ependymoglial Cells
Cerebellar Nuclei
Reticular Formation
Cranial Nerves
Olfactory Bulb
Purkinje Cells
Cochlea
Brain
Substantia Nigra
Basal Ganglia
Thalamus

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Lee, Mun Yong ; Jo, Yang Hyeok ; Chun, Myung Hoon ; Chung, Jin Woong ; Kim, Myung Suk ; Min, Do Sik. / Immunohistochemical localization of phospholipase Dl in rat central nervous system. In: Brain Research. 2000 ; Vol. 864, No. 1. pp. 52-59.
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Immunohistochemical localization of phospholipase Dl in rat central nervous system. / Lee, Mun Yong; Jo, Yang Hyeok; Chun, Myung Hoon; Chung, Jin Woong; Kim, Myung Suk; Min, Do Sik.

In: Brain Research, Vol. 864, No. 1, 05.05.2000, p. 52-59.

Research output: Contribution to journalArticle

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