Immunohistochemical study of the colonic muscle and innervation in idiopathic chronic constipation

HyoJin Park, M. A. Kamm, A. M. Abbasi, I. C. Talbot

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

PURPOSE: This study was designed to investigate neural and muscular features of the colonic wall in patients with severe idiopathic constipation. METHODS: By using quantitative immunohistochemistry, resected specimens from 14 patients with idiopathic chronic constipation and 17 nonobstructed cancer controls were studied. RESULTS: Routine histology revealed no significant histologic abnormality throughout the colon apart from four cases of melanosis coli. Ratio of the thickness of circular to longitudinal muscle was significantly lower in the left colon in constipated subjects. The myenteric plexus appeared morphologically normal in all subjects. S-100 protein, which stains neuronal supporting tissues, demonstrated an increase in the proportion of neural tissue in the myenteric plexus. There was an increased number of PGP-9.5 immunoreactive nerve fibers in the muscularis propria in constipated patients, and this was significantly higher in the ascending and descending colon. CONCLUSION: Intractably constipated patients have alterations in the neural composition of the colonic myenteric plexus and innervation of the circular muscle.

Original languageEnglish
Pages (from-to)509-513
Number of pages5
JournalDiseases of the Colon & Rectum
Volume38
Issue number5
DOIs
Publication statusPublished - 1995 May 1

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Constipation
Myenteric Plexus
Muscles
Colon
Descending Colon
Ascending Colon
Melanosis
S100 Proteins
Nerve Fibers
Histology
Coloring Agents
Immunohistochemistry
Neoplasms

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

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Immunohistochemical study of the colonic muscle and innervation in idiopathic chronic constipation. / Park, HyoJin; Kamm, M. A.; Abbasi, A. M.; Talbot, I. C.

In: Diseases of the Colon & Rectum, Vol. 38, No. 5, 01.05.1995, p. 509-513.

Research output: Contribution to journalArticle

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AB - PURPOSE: This study was designed to investigate neural and muscular features of the colonic wall in patients with severe idiopathic constipation. METHODS: By using quantitative immunohistochemistry, resected specimens from 14 patients with idiopathic chronic constipation and 17 nonobstructed cancer controls were studied. RESULTS: Routine histology revealed no significant histologic abnormality throughout the colon apart from four cases of melanosis coli. Ratio of the thickness of circular to longitudinal muscle was significantly lower in the left colon in constipated subjects. The myenteric plexus appeared morphologically normal in all subjects. S-100 protein, which stains neuronal supporting tissues, demonstrated an increase in the proportion of neural tissue in the myenteric plexus. There was an increased number of PGP-9.5 immunoreactive nerve fibers in the muscularis propria in constipated patients, and this was significantly higher in the ascending and descending colon. CONCLUSION: Intractably constipated patients have alterations in the neural composition of the colonic myenteric plexus and innervation of the circular muscle.

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