Abstract
Many polysaccharides obtained from natural sources are considered to be biological response modifiers and have been shown to enhance various immune responses. Here, we investigated the immunomodulating effects of arabinogalactan (AG) and fucoidan (FU) in vitro. Mouse spleen lymphocytes became cytotoxic to tumor cells after culture with AG and FU at concentrations of 10-100 μg/mL. Also, AG and FU were mitogenic in spleen lymphocytes and peripheral macrophages. Macrophages treated with AG and FU (10-100 μg/mL) exhibited induced tumoricidal activity and increased phagocytosis, lysosomal enzyme activity, and production of nitrite, H2O2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6. However, AG and FU had little effect on the level of IL-1β. Thus, the tumoricidal effect of AG- and FU-activated macrophages appeared to be mainly mediated by production of free radicals (NO and H2O2) and cytokines (TNF-α and IL-6). These data suggest that AG and FU are activators of lymphocytes and macrophages. This property may contribute to their effectiveness in the immunoprevention of cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 446-453 |
| Number of pages | 8 |
| Journal | Journal of medicinal food |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2005 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Medicine (miscellaneous)
- Nutrition and Dietetics
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Immunomodulating activity of arabinogalactan and focoidan in vitro. / Choi, Eun Mi; Kim, Ah Jin; Kim, Yeon O.; Hwang, Jae Kwan.
In: Journal of medicinal food, Vol. 8, No. 4, 01.12.2005, p. 446-453.Research output: Contribution to journal › Article
TY - JOUR
T1 - Immunomodulating activity of arabinogalactan and focoidan in vitro
AU - Choi, Eun Mi
AU - Kim, Ah Jin
AU - Kim, Yeon O.
AU - Hwang, Jae Kwan
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Many polysaccharides obtained from natural sources are considered to be biological response modifiers and have been shown to enhance various immune responses. Here, we investigated the immunomodulating effects of arabinogalactan (AG) and fucoidan (FU) in vitro. Mouse spleen lymphocytes became cytotoxic to tumor cells after culture with AG and FU at concentrations of 10-100 μg/mL. Also, AG and FU were mitogenic in spleen lymphocytes and peripheral macrophages. Macrophages treated with AG and FU (10-100 μg/mL) exhibited induced tumoricidal activity and increased phagocytosis, lysosomal enzyme activity, and production of nitrite, H2O2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6. However, AG and FU had little effect on the level of IL-1β. Thus, the tumoricidal effect of AG- and FU-activated macrophages appeared to be mainly mediated by production of free radicals (NO and H2O2) and cytokines (TNF-α and IL-6). These data suggest that AG and FU are activators of lymphocytes and macrophages. This property may contribute to their effectiveness in the immunoprevention of cancer.
AB - Many polysaccharides obtained from natural sources are considered to be biological response modifiers and have been shown to enhance various immune responses. Here, we investigated the immunomodulating effects of arabinogalactan (AG) and fucoidan (FU) in vitro. Mouse spleen lymphocytes became cytotoxic to tumor cells after culture with AG and FU at concentrations of 10-100 μg/mL. Also, AG and FU were mitogenic in spleen lymphocytes and peripheral macrophages. Macrophages treated with AG and FU (10-100 μg/mL) exhibited induced tumoricidal activity and increased phagocytosis, lysosomal enzyme activity, and production of nitrite, H2O2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6. However, AG and FU had little effect on the level of IL-1β. Thus, the tumoricidal effect of AG- and FU-activated macrophages appeared to be mainly mediated by production of free radicals (NO and H2O2) and cytokines (TNF-α and IL-6). These data suggest that AG and FU are activators of lymphocytes and macrophages. This property may contribute to their effectiveness in the immunoprevention of cancer.
UR - http://www.scopus.com/inward/record.url?scp=31044444853&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31044444853&partnerID=8YFLogxK
U2 - 10.1089/jmf.2005.8.446
DO - 10.1089/jmf.2005.8.446
M3 - Article
C2 - 16379554
AN - SCOPUS:31044444853
VL - 8
SP - 446
EP - 453
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
SN - 1096-620X
IS - 4
ER -