Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Guérin: a preliminary study in a rabbit model

Jong Doo Lee, Kyu Ho Shin, Sang Nae Cho, Jun Soo Shin, Min Geol Lee, Woo Ik Yang, Chang Yoon Park, Hyung Sik Yoo, Jong Tae Lee, Ok Doo Awh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Immunoscintigraphy with radiolabelled monoclonal antibodies is widely used to detect solid tumours, but only a few trials have been carried out concerning the specific in vivo localization of an inflammatory process. The purpose of this study was to investigate the detectability of tuberculous foci utilizing this method with radiolabelled bacillus Calmette-Guérin (BCG)-specific F(ab′)2 in rabbits. All of the tuberculous lesions (n=8) were clearly visualized on serial scintigraphy for up to 48 h after injection of the antibody. Immunohistochemical and Ziel-Neelson staining of the tuberculous lesions confirmed the presence of the tuberculous antigens and bacilli. It failed to demonstrate any sustained retention of the BCG-specific antibody fragment in the control group with syphilitic orchitis (n=2). Therefore, the specific in vivo localization of tuberculosis is feasible by immunoscintigraphy.

Original languageEnglish
Pages (from-to)1011-1015
Number of pages5
JournalEuropean Journal Of Nuclear Medicine
Volume19
Issue number12
DOIs
Publication statusPublished - 1992 Dec 1

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Guérin: a preliminary study in a rabbit model'. Together they form a unique fingerprint.

  • Cite this