Immunostick assay for medical diagnosis of rheumatoid arthritis

Hyuk Ko; Eun Hang Lee; Byoung Jin Jeon; Hye Jin Choi; Min Jung Kang; Jae Chul Pyun

doi:10.1007/s12257-011-0106-7

Immunostick assay for medical diagnosis of rheumatoid arthritis

Hyuk Ko, Eun Hang Lee, Byoung Jin Jeon, Hye Jin Choi, Min Jung Kang, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method.

Original language	English
Pages (from-to)	1248-1253
Number of pages	6
Journal	Biotechnology and Bioprocess Engineering
Volume	16
Issue number	6
DOIs	https://doi.org/10.1007/s12257-011-0106-7
Publication status	Published - 2011 Dec

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0082188, 2009-0073809, 2009-008-1529 and 2011-0020285) and by “Happy Tech” program (2010-0020772 and 2010-0020767).

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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title = "Immunostick assay for medical diagnosis of rheumatoid arthritis",

abstract = "An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method.",

author = "Hyuk Ko and Lee, {Eun Hang} and Jeon, {Byoung Jin} and Choi, {Hye Jin} and Kang, {Min Jung} and Pyun, {Jae Chul}",

note = "Funding Information: This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0082188, 2009-0073809, 2009-008-1529 and 2011-0020285) and by “Happy Tech” program (2010-0020772 and 2010-0020767).",

year = "2011",

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doi = "10.1007/s12257-011-0106-7",

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AU - Ko, Hyuk

AU - Lee, Eun Hang

AU - Jeon, Byoung Jin

AU - Choi, Hye Jin

AU - Kang, Min Jung

AU - Pyun, Jae Chul

N1 - Funding Information: This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0082188, 2009-0073809, 2009-008-1529 and 2011-0020285) and by “Happy Tech” program (2010-0020772 and 2010-0020767).

PY - 2011/12

Y1 - 2011/12

N2 - An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method.

AB - An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method.

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SN - 1226-8372

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ER -

Immunostick assay for medical diagnosis of rheumatoid arthritis

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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