Immunostick assay for medical diagnosis of rheumatoid arthritis

Hyuk Ko, Eun Hang Lee, Byoung Jin Jeon, Hye Jin Choi, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method.

Original languageEnglish
Pages (from-to)1248-1253
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume16
Issue number6
DOIs
Publication statusPublished - 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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