Abstract
An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method.
Original language | English |
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Pages (from-to) | 1248-1253 |
Number of pages | 6 |
Journal | Biotechnology and Bioprocess Engineering |
Volume | 16 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2011 Dec |
Bibliographical note
Funding Information:This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0082188, 2009-0073809, 2009-008-1529 and 2011-0020285) and by “Happy Tech” program (2010-0020772 and 2010-0020767).
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering