Immunostimulation and anti-DNA antibody production by backbone modified CpG-DNA

Dongbum Kim, Jae Won Rhee, Sanghoon Kwon, Wern Joo Sohn, Younghee Lee, Dae Won Kim, Doo Sik Kim, Hyung Joo Kwon

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Oligodeoxynucleotides containing immunostimulatory CpG motifs (CpG-DNA) have gained attention as potentially useful therapeutics. However, the phosphorothioate-modified CpG-DNAs (PS-ODN) can induce backbone-related side effects. Here, we compared the immunostimulatory activity of natural phosphodiester CpG-DNA (PO-ODN) from Mycobacterium bovis and PS-ODN in mice. Both PO-ODN and PS-ODN induced production of IL-12. PS-ODN increased spleen weights, spleen cell numbers, and the migration of macrophages into the peritoneal cavity in the mice in a CG sequence-dependent manner. PS-ODN induced anti-PS-ODN antibody production in the mice, and the PS-ODN-specific IgM was cross-reactive with other PS-ODNs in a CG sequence-independent manner. In contrast, PO-ODN did not affect on spleen weights, cell numbers, or IgM production. These results may provide an explanation for the side effects in immunotherapeutic application of PS-ODN. They also suggest that PO-ODN may be more optimal than PS-ODN to enhance innate immune responses without severe side effects.

Original languageEnglish
Pages (from-to)362-367
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume379
Issue number2
DOIs
Publication statusPublished - 2009 Feb 6

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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