Immunotherapy of malignant melanoma with tumor lysate-pulsed autologous monocyte-derived dendritic cells

Dae Suk Kim, Dong Hyun Kim, Boncheol Goo, Young Hun Cho, Jin Mo Park, Tae Hyung Lee, Hyun Ok Kim, Han Soo Kim, Hyunah Lee, Jong Doo Lee, Dashlkhumbe Byamba, Jeong Hwan Je, Min Geol Lee

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Abstract

Purpose: Dendritic cell (DC) vaccination for melanoma was introduced because melanoma carries distinct tumor-associated antigens. The purpose of this study was to investigate the efficacy and safety of DC vaccination for melanoma in Korea. Materials and Methods: Five patients with stage IV and one with stage II were enrolled. Autologous monocyte-derived DCs (MoDCs) were cultured and pulsed with tumor-lysate, keyhole limpet hemocyanin, and cytokine cocktail for mature antigen-loaded DC. DC vaccination was repeated four times at 2-week intervals and 2-4×10 7 DC were injected each time. Results: Reduced tumor volume was observed by PET-CT in three patients after DC vaccination. Delayed type hypersensitivity responses against tumor antigen were induced in five patients. Tumor antigen-specific IFN-γ-producing peripheral blood mononuclear cells were detected with enzyme-linked immunosorbent spot in two patients. However, the overall clinical outcome showed disease progression in all patients. Conclusion: In this study, DC vaccination using tumor antigen-loaded, mature MoDCs led to tumor regression in individual melanoma patients. Further standardization of DC vaccination protocol is required to determine which parameters lead to better anti-tumor responses and clinical outcomes.

Original languageEnglish
Pages (from-to)990-998
Number of pages9
JournalYonsei medical journal
Volume52
Issue number6
DOIs
Publication statusPublished - 2011 Nov

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Kim, D. S., Kim, D. H., Goo, B., Cho, Y. H., Park, J. M., Lee, T. H., Kim, H. O., Kim, H. S., Lee, H., Lee, J. D., Byamba, D., Je, J. H., & Lee, M. G. (2011). Immunotherapy of malignant melanoma with tumor lysate-pulsed autologous monocyte-derived dendritic cells. Yonsei medical journal, 52(6), 990-998. https://doi.org/10.3349/ymj.2011.52.6.990