Impact of pH control and heat pre-treatment of seed inoculum in dark H2 fermentation: A feasibility report using mixed microalgae biomass as feedstock

Gopalakrishnan Kumar, Guangyin Zhen, Takuro Kobayashi, Periyasamy Sivagurunathan, Sang Hyoun Kim, Kai Qin Xu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

This study investigated the effect of controlling pH (5.5) and heat pre-treatment of seed inoculum in dark fermentative hydrogen production. The results showed that only inoculum source plays an important role rather than pH and heat treatment. Seed source is vital factor despite, heat treatment and pH controlled at 5.5. Mesophilic fermentation resulted in CH4 generation, however, thermophilic fermentation while using thermophilic inoculum is opted for H2 generation. In contrast promoted mesophilic inoculum (mesophilic to thermophilic) still documented for CH4 generation. Peak hydrogen production rate (HPR) and methane production rate (MPR) were noted as 90 and 117 mL/L-d, during the conditions of thermo inoculum (thermophilic, pH 5.5) and pH no control (mesophilic) experiments, respectively. Peak, total solids (TS) and chemical oxygen demand (COD) removal were achieved as 56 and 42% at mesophilic condition. Volatile fatty acid (VFA) profiling revealed the background of the process performances. Microbial community analysis via fluorescent in-situ hybridization (FISH) narrated that bacteria and archaea communities were enriched during thermophilic and mesophilic experiments, respectively. Besides, the presence of methanogens revealed that heat treatment and controlling moderately acidic pH (5.5) could not completely eliminate them and resulted in CH4 generation, rather than H2 production.

Original languageEnglish
Pages (from-to)4382-4392
Number of pages11
JournalInternational Journal of Hydrogen Energy
Volume41
Issue number7
DOIs
Publication statusPublished - 2016 Feb 23

Fingerprint

inoculum
fermentation
biomass
pretreatment
Fermentation
Feedstocks
Seed
seeds
Biomass
Heat treatment
Hydrogen production
heat
Methanogens
Volatile fatty acids
heat treatment
Chemical oxygen demand
hydrogen production
Bacteria
Methane
Experiments

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

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title = "Impact of pH control and heat pre-treatment of seed inoculum in dark H2 fermentation: A feasibility report using mixed microalgae biomass as feedstock",
abstract = "This study investigated the effect of controlling pH (5.5) and heat pre-treatment of seed inoculum in dark fermentative hydrogen production. The results showed that only inoculum source plays an important role rather than pH and heat treatment. Seed source is vital factor despite, heat treatment and pH controlled at 5.5. Mesophilic fermentation resulted in CH4 generation, however, thermophilic fermentation while using thermophilic inoculum is opted for H2 generation. In contrast promoted mesophilic inoculum (mesophilic to thermophilic) still documented for CH4 generation. Peak hydrogen production rate (HPR) and methane production rate (MPR) were noted as 90 and 117 mL/L-d, during the conditions of thermo inoculum (thermophilic, pH 5.5) and pH no control (mesophilic) experiments, respectively. Peak, total solids (TS) and chemical oxygen demand (COD) removal were achieved as 56 and 42{\%} at mesophilic condition. Volatile fatty acid (VFA) profiling revealed the background of the process performances. Microbial community analysis via fluorescent in-situ hybridization (FISH) narrated that bacteria and archaea communities were enriched during thermophilic and mesophilic experiments, respectively. Besides, the presence of methanogens revealed that heat treatment and controlling moderately acidic pH (5.5) could not completely eliminate them and resulted in CH4 generation, rather than H2 production.",
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Impact of pH control and heat pre-treatment of seed inoculum in dark H2 fermentation : A feasibility report using mixed microalgae biomass as feedstock. / Kumar, Gopalakrishnan; Zhen, Guangyin; Kobayashi, Takuro; Sivagurunathan, Periyasamy; Kim, Sang Hyoun; Xu, Kai Qin.

In: International Journal of Hydrogen Energy, Vol. 41, No. 7, 23.02.2016, p. 4382-4392.

Research output: Contribution to journalArticle

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