Impaired expression of MAPK is associated with the downregulation of TNF-α, IL-6, and IL-10 in Mycobacterium abscessus lung disease

Yun Su Sim, Su Young Kim, Eun Joo Kim, SungJae Shin, Won Jung Koh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Healthy individuals who develop nontuberculous mycobacteria (NTM) lung disease are likely to have specific susceptibility factors which can lead to a NTM infection. The aim of the present study was to investigate the mechanism underlying innate immune responses, including the role of mitogen-activated protein kinase (MAPK), in Mycobacterium abscessus lung disease. Methods: Extracellular signal-regulated kinase (ERK1/2) and p38 MAPK expression in monocytes from peripheral blood mononuclear cells were measured by Western blot analysis after stimulation by Mycobacterium avium in five patients with M. abscessus lung disease and seven healthy controls. A M. avium-induced cytokine assay was performed after inhibition of ERK1/2 and p38 MAPK pathways. Results: Mycobacterium avium induced p38 and ERK1/2 expression in monocytes from healthy controls and subsequently upregulated tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 production. In monocytes from patients with M. abscessus lung disease, however, induction of p38 and ERK1/2 expression, and the production of TNF-α, IL-6, and IL-10 were significantly lower. Conclusion: Decreased activity of MAPK and cytokine secretion in monocytes from patients with M. abscessus lung disease may provide an explanation regarding host susceptibility to these uncommon infections. Copyright

Original languageEnglish
Pages (from-to)275-283
Number of pages9
JournalTuberculosis and Respiratory Diseases
Volume72
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1

Fingerprint

Mycobacterium
Mitogen-Activated Protein Kinases
Interleukin-10
Lung Diseases
Interleukin-6
Down-Regulation
Mycobacterium avium
Tumor Necrosis Factor-alpha
Monocytes
p38 Mitogen-Activated Protein Kinases
Nontuberculous Mycobacterium Infections
Cytokines
Nontuberculous Mycobacteria
Mitogen-Activated Protein Kinase 1
Innate Immunity
Blood Cells
Western Blotting
Infection

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

@article{9c8cc11ba7db4a96b592ec8ab12ad03c,
title = "Impaired expression of MAPK is associated with the downregulation of TNF-α, IL-6, and IL-10 in Mycobacterium abscessus lung disease",
abstract = "Background: Healthy individuals who develop nontuberculous mycobacteria (NTM) lung disease are likely to have specific susceptibility factors which can lead to a NTM infection. The aim of the present study was to investigate the mechanism underlying innate immune responses, including the role of mitogen-activated protein kinase (MAPK), in Mycobacterium abscessus lung disease. Methods: Extracellular signal-regulated kinase (ERK1/2) and p38 MAPK expression in monocytes from peripheral blood mononuclear cells were measured by Western blot analysis after stimulation by Mycobacterium avium in five patients with M. abscessus lung disease and seven healthy controls. A M. avium-induced cytokine assay was performed after inhibition of ERK1/2 and p38 MAPK pathways. Results: Mycobacterium avium induced p38 and ERK1/2 expression in monocytes from healthy controls and subsequently upregulated tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 production. In monocytes from patients with M. abscessus lung disease, however, induction of p38 and ERK1/2 expression, and the production of TNF-α, IL-6, and IL-10 were significantly lower. Conclusion: Decreased activity of MAPK and cytokine secretion in monocytes from patients with M. abscessus lung disease may provide an explanation regarding host susceptibility to these uncommon infections. Copyright",
author = "Sim, {Yun Su} and Kim, {Su Young} and Kim, {Eun Joo} and SungJae Shin and Koh, {Won Jung}",
year = "2012",
month = "3",
day = "1",
doi = "10.4046/trd.2012.72.3.275",
language = "English",
volume = "72",
pages = "275--283",
journal = "Tuberculosis and Respiratory Diseases",
issn = "1738-3536",
publisher = "The Korean Academy of Tuberculosis and Respiratory Diseases",
number = "3",

}

Impaired expression of MAPK is associated with the downregulation of TNF-α, IL-6, and IL-10 in Mycobacterium abscessus lung disease. / Sim, Yun Su; Kim, Su Young; Kim, Eun Joo; Shin, SungJae; Koh, Won Jung.

In: Tuberculosis and Respiratory Diseases, Vol. 72, No. 3, 01.03.2012, p. 275-283.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Impaired expression of MAPK is associated with the downregulation of TNF-α, IL-6, and IL-10 in Mycobacterium abscessus lung disease

AU - Sim, Yun Su

AU - Kim, Su Young

AU - Kim, Eun Joo

AU - Shin, SungJae

AU - Koh, Won Jung

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Background: Healthy individuals who develop nontuberculous mycobacteria (NTM) lung disease are likely to have specific susceptibility factors which can lead to a NTM infection. The aim of the present study was to investigate the mechanism underlying innate immune responses, including the role of mitogen-activated protein kinase (MAPK), in Mycobacterium abscessus lung disease. Methods: Extracellular signal-regulated kinase (ERK1/2) and p38 MAPK expression in monocytes from peripheral blood mononuclear cells were measured by Western blot analysis after stimulation by Mycobacterium avium in five patients with M. abscessus lung disease and seven healthy controls. A M. avium-induced cytokine assay was performed after inhibition of ERK1/2 and p38 MAPK pathways. Results: Mycobacterium avium induced p38 and ERK1/2 expression in monocytes from healthy controls and subsequently upregulated tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 production. In monocytes from patients with M. abscessus lung disease, however, induction of p38 and ERK1/2 expression, and the production of TNF-α, IL-6, and IL-10 were significantly lower. Conclusion: Decreased activity of MAPK and cytokine secretion in monocytes from patients with M. abscessus lung disease may provide an explanation regarding host susceptibility to these uncommon infections. Copyright

AB - Background: Healthy individuals who develop nontuberculous mycobacteria (NTM) lung disease are likely to have specific susceptibility factors which can lead to a NTM infection. The aim of the present study was to investigate the mechanism underlying innate immune responses, including the role of mitogen-activated protein kinase (MAPK), in Mycobacterium abscessus lung disease. Methods: Extracellular signal-regulated kinase (ERK1/2) and p38 MAPK expression in monocytes from peripheral blood mononuclear cells were measured by Western blot analysis after stimulation by Mycobacterium avium in five patients with M. abscessus lung disease and seven healthy controls. A M. avium-induced cytokine assay was performed after inhibition of ERK1/2 and p38 MAPK pathways. Results: Mycobacterium avium induced p38 and ERK1/2 expression in monocytes from healthy controls and subsequently upregulated tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 production. In monocytes from patients with M. abscessus lung disease, however, induction of p38 and ERK1/2 expression, and the production of TNF-α, IL-6, and IL-10 were significantly lower. Conclusion: Decreased activity of MAPK and cytokine secretion in monocytes from patients with M. abscessus lung disease may provide an explanation regarding host susceptibility to these uncommon infections. Copyright

UR - http://www.scopus.com/inward/record.url?scp=84867467328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867467328&partnerID=8YFLogxK

U2 - 10.4046/trd.2012.72.3.275

DO - 10.4046/trd.2012.72.3.275

M3 - Article

C2 - 23227067

AN - SCOPUS:84867467328

VL - 72

SP - 275

EP - 283

JO - Tuberculosis and Respiratory Diseases

JF - Tuberculosis and Respiratory Diseases

SN - 1738-3536

IS - 3

ER -