Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas

Kwi H. Koh, Hyun J. Kang, Long S. Li, Nam Gyun Kim, Kwon T. You, Eungi Yang, Hyunki Kim, Heejin Kim, Chae Ok Yun, Kyung Sup Kim, Hoguen Kim

Research output: Contribution to journalArticle

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Abstract

Frameshift mutations of coding mononucleotide repeat of the hRAD50 gene and formation of the mutant hMRE11 splicing variant are frequent events in tumors with mismatch repair (MMR) deficiency. Both the hRAD50 and hMRE11 proteins form a heterotrimer with the NBS1, and this heterotrimer is involved in the double strand DNA break repair by homologous recombination and nonhomologous end-joining (NHEJ). In order to clarify the role of hRAD50 and hMRE11 gene alterations in MMR-deficient tumors, we analyzed the expression of the hRAD50 and hMRE11 proteins and we evaluated NHEJ in the seven MMR-deficient and five MMR-proficient colon cancer cell lines. Frameshift mutations of the hRAD50 gene were found in five of seven MMR-deficient cell lines, and this was directly related to the decreased expression of hRAD50 mRNA and protein. The mutant hMRE11 splicing variant was found in all of the seven MMR-deficient cell lines, and this was related to the decreased hMRE11 expression in four of the seven MMR-deficient cell lines. MMR-deficient cell lines with decreased hRAD50 and hMRE11 expressions were more sensitive to γ-irradiation, and these cell lines showed an impaired NHEJ. The impairment of NHEJ was induced after knockdown of hRAD50 and hMRE11 through small interference RNA. Our findings suggest that mutations of hRAD50 and hMRE11 genes in MMR-deficient tumors are related to the defects in NHEJ, and this may result in chromosomal changes during the progression of tumor.

Original languageEnglish
Pages (from-to)1130-1138
Number of pages9
JournalLaboratory Investigation
Volume85
Issue number9
DOIs
Publication statusPublished - 2005 Dec 1

Fingerprint

DNA Mismatch Repair
Colon
Carcinoma
Cell Line
Frameshift Mutation
Genes
Neoplasms
Proteins
Double-Stranded DNA Breaks
Homologous Recombination
RNA Interference
DNA Repair
Colonic Neoplasms
Messenger RNA
Mutation

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Koh, K. H., Kang, H. J., Li, L. S., Kim, N. G., You, K. T., Yang, E., ... Kim, H. (2005). Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas. Laboratory Investigation, 85(9), 1130-1138. https://doi.org/10.1038/labinvest.3700315
Koh, Kwi H. ; Kang, Hyun J. ; Li, Long S. ; Kim, Nam Gyun ; You, Kwon T. ; Yang, Eungi ; Kim, Hyunki ; Kim, Heejin ; Yun, Chae Ok ; Kim, Kyung Sup ; Kim, Hoguen. / Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas. In: Laboratory Investigation. 2005 ; Vol. 85, No. 9. pp. 1130-1138.
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abstract = "Frameshift mutations of coding mononucleotide repeat of the hRAD50 gene and formation of the mutant hMRE11 splicing variant are frequent events in tumors with mismatch repair (MMR) deficiency. Both the hRAD50 and hMRE11 proteins form a heterotrimer with the NBS1, and this heterotrimer is involved in the double strand DNA break repair by homologous recombination and nonhomologous end-joining (NHEJ). In order to clarify the role of hRAD50 and hMRE11 gene alterations in MMR-deficient tumors, we analyzed the expression of the hRAD50 and hMRE11 proteins and we evaluated NHEJ in the seven MMR-deficient and five MMR-proficient colon cancer cell lines. Frameshift mutations of the hRAD50 gene were found in five of seven MMR-deficient cell lines, and this was directly related to the decreased expression of hRAD50 mRNA and protein. The mutant hMRE11 splicing variant was found in all of the seven MMR-deficient cell lines, and this was related to the decreased hMRE11 expression in four of the seven MMR-deficient cell lines. MMR-deficient cell lines with decreased hRAD50 and hMRE11 expressions were more sensitive to γ-irradiation, and these cell lines showed an impaired NHEJ. The impairment of NHEJ was induced after knockdown of hRAD50 and hMRE11 through small interference RNA. Our findings suggest that mutations of hRAD50 and hMRE11 genes in MMR-deficient tumors are related to the defects in NHEJ, and this may result in chromosomal changes during the progression of tumor.",
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Koh, KH, Kang, HJ, Li, LS, Kim, NG, You, KT, Yang, E, Kim, H, Kim, H, Yun, CO, Kim, KS & Kim, H 2005, 'Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas', Laboratory Investigation, vol. 85, no. 9, pp. 1130-1138. https://doi.org/10.1038/labinvest.3700315

Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas. / Koh, Kwi H.; Kang, Hyun J.; Li, Long S.; Kim, Nam Gyun; You, Kwon T.; Yang, Eungi; Kim, Hyunki; Kim, Heejin; Yun, Chae Ok; Kim, Kyung Sup; Kim, Hoguen.

In: Laboratory Investigation, Vol. 85, No. 9, 01.12.2005, p. 1130-1138.

Research output: Contribution to journalArticle

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T1 - Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas

AU - Koh, Kwi H.

AU - Kang, Hyun J.

AU - Li, Long S.

AU - Kim, Nam Gyun

AU - You, Kwon T.

AU - Yang, Eungi

AU - Kim, Hyunki

AU - Kim, Heejin

AU - Yun, Chae Ok

AU - Kim, Kyung Sup

AU - Kim, Hoguen

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N2 - Frameshift mutations of coding mononucleotide repeat of the hRAD50 gene and formation of the mutant hMRE11 splicing variant are frequent events in tumors with mismatch repair (MMR) deficiency. Both the hRAD50 and hMRE11 proteins form a heterotrimer with the NBS1, and this heterotrimer is involved in the double strand DNA break repair by homologous recombination and nonhomologous end-joining (NHEJ). In order to clarify the role of hRAD50 and hMRE11 gene alterations in MMR-deficient tumors, we analyzed the expression of the hRAD50 and hMRE11 proteins and we evaluated NHEJ in the seven MMR-deficient and five MMR-proficient colon cancer cell lines. Frameshift mutations of the hRAD50 gene were found in five of seven MMR-deficient cell lines, and this was directly related to the decreased expression of hRAD50 mRNA and protein. The mutant hMRE11 splicing variant was found in all of the seven MMR-deficient cell lines, and this was related to the decreased hMRE11 expression in four of the seven MMR-deficient cell lines. MMR-deficient cell lines with decreased hRAD50 and hMRE11 expressions were more sensitive to γ-irradiation, and these cell lines showed an impaired NHEJ. The impairment of NHEJ was induced after knockdown of hRAD50 and hMRE11 through small interference RNA. Our findings suggest that mutations of hRAD50 and hMRE11 genes in MMR-deficient tumors are related to the defects in NHEJ, and this may result in chromosomal changes during the progression of tumor.

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