Impedimetric analysis on the mass transfer properties of intact and competent E. coli cells

Youngjoon Lim, Chun Hao Su, Ying Chih Liao, Sangyup Lee

Research output: Contribution to journalArticle

Abstract

Competent Escherichia coli cells are commonly used in bacterial transformation owing to its high permeability for bioorganic macromolecules like plasmid DNA. However, the mass transfer property of competent E. coli cell has not fully investigated. In the present study, mass transfer coefficients of competent and intact E. coli cells in deionized water were evaluated by impedimetric analysis of the release of cytoplasmic compounds. Because competent cells have a higher permeability after chemical treatment, the lumped mass transfer coefficient of a competent cell was approximately 6.5 times larger than that of an intact cell at room temperature. Release of cytoplasmic components was accelerated at an elevated temperature of 42 °C, which is the heat shock temperature used during bacterial transformation. At this elevated temperature, assessed lumped mass transfer coefficients of intact and competent E. coli cells were 9.28 × 10−4 min−1 and 97.10 × 10−4 min−1, respectively. Significant increase in the mass transfer coefficient of the competent cell is caused by cytolysis of cells. The double layer capacitances were also assessed from the electrochemical spectra confirming the enhanced ion release from E. coli cells and rupture of the competent cell under prolonged exposure at the elevated temperature. Impedimetric detection of the ion release with analyses using an equivalent circuit model provides a method to evaluate mass transfer properties of biomolecules.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1861
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

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Escherichia coli
Mass transfer
Bacterial Transformation
Temperature
Ions
Deionized water
Biomolecules
Macromolecules
Equivalent circuits
Permeability
Plasmids
Capacitance
DNA
Rupture
Shock
Hot Temperature
Water

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

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Impedimetric analysis on the mass transfer properties of intact and competent E. coli cells. / Lim, Youngjoon; Su, Chun Hao; Liao, Ying Chih; Lee, Sangyup.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1861, No. 1, 01.01.2019, p. 9-16.

Research output: Contribution to journalArticle

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