Impedimetric tumor necrosis factor-α sensor based on a reduced graphene oxide nanoparticle-modified electrode array

Ajay Kumar Yagati, Ga Yeon Lee, Sungji Ha, Keun A. Chang, Jae Chul Pyun, Sungbo Cho

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A surface was prepared by simple electrodeposition of reduced graphene oxide (RGO) coated with gold nanoparticles (AuNP) on an indium tin oxide microelectrode array, and its ability and applicability in a tumor necrosis factor-alpha (TNF-α) detection sensor was evaluated by electrochemical impedance spectroscopy (EIS). The AuNP-RGO hybrid structure showed improved electrical conductivity when compared with the conductivities of its individual elements and demonstrated an excellent capturing ability for TNF-α antibody-antigen binding. A quantitative determination of the TNF-α detection performance was achieved by measuring the resistance changes with varying TNF-α concentrations while using a [Fe(CN)6]3-/4- redox probe in EIS. The antibody covered electrode resistance increases approximately linearly (r = 0.969) with increasing antigen concentration. The proposed TNF-α sensor has a 0.43 pg mL-1 minimum detection limit and a linear range of 1-1000 pg mL-1.

Original languageEnglish
Pages (from-to)11921-11927
Number of pages7
JournalJournal of Nanoscience and Nanotechnology
Volume16
Issue number11
DOIs
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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