Impedometric estrogen biosensor based on estrogen receptor alpha-immobilized gold electrode

Byung Kun Kim, Jing Li, Ji Eun Im, Kwang Soo Ahn, Tu San Park, Seong In Cho, Yong Rok Kim, Wonyong Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A label-free impedometric biosensor has been developed for the detection of estrogen hormone 17β-estradiol. The electrochemical biosensor has been fabricated by immobilizing both estrogen receptor-α and bovine serum albumin on gold electrode surfaces. The binding of 17β-estradiol to the estrogen receptors on the fabricated biosensor has increased the electron-transfer resistance which has been directly monitored by electrochemical impedance spectroscopy in the presence of 5.0 mM K 3Fe(CN)6/K4Fe(CN)6 (1:1, v/v) redox couple. The association constant between the 17β-estradiol and the estrogen receptors has been found to be ca. 1.8 × 1011 M-1, which indicates that the estrogen receptors are well formed on gold electrode surfaces not only with high density but also with good specificity to its corresponding 17β-estradiol. The present biosensor gives a linear response (r2 = 0.992) for 17β-estradiol concentration from 1.0 × 10-13 to 1.0 × 10-9 M with a detection limit of 1.0 × 10-13 M (S/N = 3), which is much lower compared to those obtained with other detection methods. The present biosensor exhibits good stability, in which the biosensor has retained 88% of its initial activity after 3 weeks of storage in 50 mM phosphate buffer at pH 7.0.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalJournal of Electroanalytical Chemistry
Volume671
DOIs
Publication statusPublished - 2012 Apr 15

Fingerprint

Estrogen Receptor alpha
Biosensors
Gold
Estrogens
Estradiol
Estrogen Receptors
Electrodes
Hormones
Bovine Serum Albumin
Electrochemical impedance spectroscopy
Labels
Buffers
Phosphates
Association reactions
Electrons

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

Cite this

Kim, Byung Kun ; Li, Jing ; Im, Ji Eun ; Ahn, Kwang Soo ; Park, Tu San ; Cho, Seong In ; Kim, Yong Rok ; Lee, Wonyong. / Impedometric estrogen biosensor based on estrogen receptor alpha-immobilized gold electrode. In: Journal of Electroanalytical Chemistry. 2012 ; Vol. 671. pp. 106-111.
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abstract = "A label-free impedometric biosensor has been developed for the detection of estrogen hormone 17β-estradiol. The electrochemical biosensor has been fabricated by immobilizing both estrogen receptor-α and bovine serum albumin on gold electrode surfaces. The binding of 17β-estradiol to the estrogen receptors on the fabricated biosensor has increased the electron-transfer resistance which has been directly monitored by electrochemical impedance spectroscopy in the presence of 5.0 mM K 3Fe(CN)6/K4Fe(CN)6 (1:1, v/v) redox couple. The association constant between the 17β-estradiol and the estrogen receptors has been found to be ca. 1.8 × 1011 M-1, which indicates that the estrogen receptors are well formed on gold electrode surfaces not only with high density but also with good specificity to its corresponding 17β-estradiol. The present biosensor gives a linear response (r2 = 0.992) for 17β-estradiol concentration from 1.0 × 10-13 to 1.0 × 10-9 M with a detection limit of 1.0 × 10-13 M (S/N = 3), which is much lower compared to those obtained with other detection methods. The present biosensor exhibits good stability, in which the biosensor has retained 88{\%} of its initial activity after 3 weeks of storage in 50 mM phosphate buffer at pH 7.0.",
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Impedometric estrogen biosensor based on estrogen receptor alpha-immobilized gold electrode. / Kim, Byung Kun; Li, Jing; Im, Ji Eun; Ahn, Kwang Soo; Park, Tu San; Cho, Seong In; Kim, Yong Rok; Lee, Wonyong.

In: Journal of Electroanalytical Chemistry, Vol. 671, 15.04.2012, p. 106-111.

Research output: Contribution to journalArticle

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AU - Cho, Seong In

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