A label-free impedometric biosensor has been developed for the detection of estrogen hormone 17β-estradiol. The electrochemical biosensor has been fabricated by immobilizing both estrogen receptor-α and bovine serum albumin on gold electrode surfaces. The binding of 17β-estradiol to the estrogen receptors on the fabricated biosensor has increased the electron-transfer resistance which has been directly monitored by electrochemical impedance spectroscopy in the presence of 5.0 mM K 3Fe(CN)6/K4Fe(CN)6 (1:1, v/v) redox couple. The association constant between the 17β-estradiol and the estrogen receptors has been found to be ca. 1.8 × 1011 M-1, which indicates that the estrogen receptors are well formed on gold electrode surfaces not only with high density but also with good specificity to its corresponding 17β-estradiol. The present biosensor gives a linear response (r2 = 0.992) for 17β-estradiol concentration from 1.0 × 10-13 to 1.0 × 10-9 M with a detection limit of 1.0 × 10-13 M (S/N = 3), which is much lower compared to those obtained with other detection methods. The present biosensor exhibits good stability, in which the biosensor has retained 88% of its initial activity after 3 weeks of storage in 50 mM phosphate buffer at pH 7.0.
Bibliographical noteFunding Information:
This study was supported by a Grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs (A085136) and the Grant of the National Research Foundation of Korea (NRF) funded by the Korea Government (MEST) (No. 2011-0001129).
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Chemical Engineering(all)