Importance of region-specific epithelial rearrangements in mouse rugae development

Wern Joo Sohn, Hitoshi Yamamoto, Hong In Shin, Zae Young Ryoo, Sanggyu Lee, Yong Chul Bae, Hansung Jung, Jae Young Kim

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10 Citations (Scopus)

Abstract

Epithelial appendages on palatal rugae develop during mouse palatogenesis through epithelial thickening and pattern formation. Recently, the patterned formation of nine rugae was observed together with the specific expression patterns of Shh in rodents. However, no crucial evidence was found for a direct association between Shh expression and the distinct structural formation of rugae. In order to reveal possible relationships, we investigated the morphological changes of rugae and expression patterns of Shh directly by in vitro organ culture at embryonic day 13 (E13) for 2 days. To compare and examine the diverse growing aspects of the palate and rugae, we carefully observed the detailed morphogenesis, with cell proliferation of the rugae occurring between E13 and E14.5. After 2 days of cultivation at E13, DiI micro-injections revealed that the middle part of the palate, adjacent to the upper molar-forming region, contributed to the formation of the subsequent structure of rugae by extensive cell rearrangement and proliferation within the epithelium in the preferred anteroposterior direction. The results also defined the intimate relationship between Shh expression and rugae formation.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalCell and Tissue Research
Volume344
Issue number2
DOIs
Publication statusPublished - 2011 May 1

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Palate
Cell Proliferation
Organ Culture Techniques
Morphogenesis
Rodentia
Epithelium
Injections
Direction compound
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Sohn, W. J., Yamamoto, H., Shin, H. I., Ryoo, Z. Y., Lee, S., Bae, Y. C., ... Kim, J. Y. (2011). Importance of region-specific epithelial rearrangements in mouse rugae development. Cell and Tissue Research, 344(2), 271-277. https://doi.org/10.1007/s00441-011-1148-z
Sohn, Wern Joo ; Yamamoto, Hitoshi ; Shin, Hong In ; Ryoo, Zae Young ; Lee, Sanggyu ; Bae, Yong Chul ; Jung, Hansung ; Kim, Jae Young. / Importance of region-specific epithelial rearrangements in mouse rugae development. In: Cell and Tissue Research. 2011 ; Vol. 344, No. 2. pp. 271-277.
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Sohn, WJ, Yamamoto, H, Shin, HI, Ryoo, ZY, Lee, S, Bae, YC, Jung, H & Kim, JY 2011, 'Importance of region-specific epithelial rearrangements in mouse rugae development', Cell and Tissue Research, vol. 344, no. 2, pp. 271-277. https://doi.org/10.1007/s00441-011-1148-z

Importance of region-specific epithelial rearrangements in mouse rugae development. / Sohn, Wern Joo; Yamamoto, Hitoshi; Shin, Hong In; Ryoo, Zae Young; Lee, Sanggyu; Bae, Yong Chul; Jung, Hansung; Kim, Jae Young.

In: Cell and Tissue Research, Vol. 344, No. 2, 01.05.2011, p. 271-277.

Research output: Contribution to journalArticle

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