Clostridium acetobutylicum is an attractive industrial microorganism for biochemical production, but there have been few attempts for bio-hydrogen production based on metabolic engineering. In this study, metabolically engineered C. acetobutylicum carrying glucose-6-phosphate dehydrogenase (zwf) and FeFe hydrogenase (hydA) were constructed as recombinant strains CA-zwf(pIMP-zwf) and CA-hydA(pMTL-hydA), respectively, to improve hydrogen productivity. The results showed that the engineered strains produced 1.15 and 1.39-fold higher hydrogen yield, respectively, than the wild type. Furthermore, when pH and glucose concentration were optimized for the CA-hydA strain, enhanced hydrogen productivity of 25.8% was achieved in 7 L jar scale fermentation. This result provides an insight into the future direction for metabolic engineering of C. acetobutylicum for improved hydrogen production.
|Number of pages||9|
|Journal||International Journal of Hydrogen Energy|
|Publication status||Published - 2021 Oct 26|
Bibliographical noteFunding Information:
This work was supported by National Research Foundation of Korea grant funded by the Korea government (Ministry of Science and ICT, MSIT) ( NRF-2019M3E6A1103839 ) and the Korea Institute of Energy Technology Evaluation and Planning and the Ministry of Trade, Industry and Energy of the Republic of Korea ( 20188550000540 ).
© 2021 Hydrogen Energy Publications LLC
All Science Journal Classification (ASJC) codes
- Renewable Energy, Sustainability and the Environment
- Fuel Technology
- Condensed Matter Physics
- Energy Engineering and Power Technology