Improved L-lysine production by the amplification of the CorynebacteriumglutamicumdapA gene encoding dihydrodipicolinate synthetase in E.coli

Jong Won Oh, Jin Ho Lee, Kap Soo Noh, Hyune Hwan Lee, Jae Heung Lee, Hyung Hwan Hyun

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The dapA gene (L-2,3-dihydrodipicolinate synthetase: DHDP synthetase) of Corynebacteriumglutamicum JS231, a lysine overproducer, cloned and subcloned in E.coli/C.glutamicum shuttle vector pECCG117 was used to transform E.coli threonine producer and threonine and lysine coproducer. The plasmid pDHDP5812 carrying dapA gene of C.glutamicum led to increase in lysine production in these E.coli strains. Threonine and lysine co-producer E.coli TF1 with pDHDP5812 produced lysine with small amount of threonine. The DHDP synthetase activity of E.coli TF1 carrying pDHDP5812 showed high resistance toward inhibition by lysine.

Original languageEnglish
Pages (from-to)727-732
Number of pages6
JournalBiotechnology Letters
Issue number10
Publication statusPublished - 1991 Oct 1


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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