Improved L-lysine production by the amplification of the CorynebacteriumglutamicumdapA gene encoding dihydrodipicolinate synthetase in E.coli

Jong-Won Oh, Jin Ho Lee, Kap Soo Noh, Hyune Hwan Lee, Jae Heung Lee, Hyung Hwan Hyun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The dapA gene (L-2,3-dihydrodipicolinate synthetase: DHDP synthetase) of Corynebacteriumglutamicum JS231, a lysine overproducer, cloned and subcloned in E.coli/C.glutamicum shuttle vector pECCG117 was used to transform E.coli threonine producer and threonine and lysine coproducer. The plasmid pDHDP5812 carrying dapA gene of C.glutamicum led to increase in lysine production in these E.coli strains. Threonine and lysine co-producer E.coli TF1 with pDHDP5812 produced lysine with small amount of threonine. The DHDP synthetase activity of E.coli TF1 carrying pDHDP5812 showed high resistance toward inhibition by lysine.

Original languageEnglish
Pages (from-to)727-732
Number of pages6
JournalBiotechnology Letters
Volume13
Issue number10
DOIs
Publication statusPublished - 1991 Oct 1

Fingerprint

Gene encoding
Gene Amplification
Ligases
Escherichia coli
Lysine
Amplification
Threonine
Genes
Genetic Vectors
Plasmids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Oh, Jong-Won ; Lee, Jin Ho ; Noh, Kap Soo ; Lee, Hyune Hwan ; Lee, Jae Heung ; Hyun, Hyung Hwan. / Improved L-lysine production by the amplification of the CorynebacteriumglutamicumdapA gene encoding dihydrodipicolinate synthetase in E.coli. In: Biotechnology Letters. 1991 ; Vol. 13, No. 10. pp. 727-732.
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abstract = "The dapA gene (L-2,3-dihydrodipicolinate synthetase: DHDP synthetase) of Corynebacteriumglutamicum JS231, a lysine overproducer, cloned and subcloned in E.coli/C.glutamicum shuttle vector pECCG117 was used to transform E.coli threonine producer and threonine and lysine coproducer. The plasmid pDHDP5812 carrying dapA gene of C.glutamicum led to increase in lysine production in these E.coli strains. Threonine and lysine co-producer E.coli TF1 with pDHDP5812 produced lysine with small amount of threonine. The DHDP synthetase activity of E.coli TF1 carrying pDHDP5812 showed high resistance toward inhibition by lysine.",
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Improved L-lysine production by the amplification of the CorynebacteriumglutamicumdapA gene encoding dihydrodipicolinate synthetase in E.coli. / Oh, Jong-Won; Lee, Jin Ho; Noh, Kap Soo; Lee, Hyune Hwan; Lee, Jae Heung; Hyun, Hyung Hwan.

In: Biotechnology Letters, Vol. 13, No. 10, 01.10.1991, p. 727-732.

Research output: Contribution to journalArticle

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AU - Hyun, Hyung Hwan

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