The dapA gene (L-2,3-dihydrodipicolinate synthetase: DHDP synthetase) of Corynebacteriumglutamicum JS231, a lysine overproducer, cloned and subcloned in E.coli/C.glutamicum shuttle vector pECCG117 was used to transform E.coli threonine producer and threonine and lysine coproducer. The plasmid pDHDP5812 carrying dapA gene of C.glutamicum led to increase in lysine production in these E.coli strains. Threonine and lysine co-producer E.coli TF1 with pDHDP5812 produced lysine with small amount of threonine. The DHDP synthetase activity of E.coli TF1 carrying pDHDP5812 showed high resistance toward inhibition by lysine.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology