Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Kyungwon Lee; Chang Ki Kim; Dongeun Yong; Seok Hoon Jeong; Jong Hwa Yum; Young Hee Seo; Jean Denis Docquier; Yunsop Chong

doi:10.1016/j.mimet.2010.08.010

Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Kyungwon Lee, Chang Ki Kim, Dongeun Yong, Seok Hoon Jeong, Jong Hwa Yum, Young Hee Seo, Jean Denis Docquier, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article

46 Citations (Scopus)

Abstract

The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.

Original language	English
Pages (from-to)	149-152
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2010.08.010
Publication status	Published - 2010 Nov 1

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All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

Cite this

Lee, K., Kim, C. K., Yong, D., Jeong, S. H., Yum, J. H., Seo, Y. H., Docquier, J. D., & Chong, Y. (2010). Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. Journal of Microbiological Methods, 83(2), 149-152. https://doi.org/10.1016/j.mimet.2010.08.010

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abstract = "The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.",

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Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. / Lee, Kyungwon; Kim, Chang Ki; Yong, Dongeun ; Jeong, Seok Hoon; Yum, Jong Hwa; Seo, Young Hee; Docquier, Jean Denis; Chong, Yunsop.

In: Journal of Microbiological Methods, Vol. 83, No. 2, 01.11.2010, p. 149-152.

Research output: Contribution to journal › Article

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AB - The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.

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10.1016/j.mimet.2010.08.010