Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Kyungwon Lee, Chang Ki Kim, DongEun Yong, Seokhoon Jeong, Jong Hwa Yum, Young Hee Seo, Jean Denis Docquier, Yunsop Chong

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.

Original languageEnglish
Pages (from-to)149-152
Number of pages4
JournalJournal of Microbiological Methods
Volume83
Issue number2
DOIs
Publication statusPublished - 2010 Nov 1

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Bacillus
Agar
Bile
carbapenemase
Therapeutics
Asp(5)-oxytocin

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

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abstract = "The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.",
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Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. / Lee, Kyungwon; Kim, Chang Ki; Yong, DongEun; Jeong, Seokhoon; Yum, Jong Hwa; Seo, Young Hee; Docquier, Jean Denis; Chong, Yunsop.

In: Journal of Microbiological Methods, Vol. 83, No. 2, 01.11.2010, p. 149-152.

Research output: Contribution to journalArticle

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AU - Yum, Jong Hwa

AU - Seo, Young Hee

AU - Docquier, Jean Denis

AU - Chong, Yunsop

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