Abstract
The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.
| Original language | English |
|---|---|
| Pages (from-to) | 149-152 |
| Number of pages | 4 |
| Journal | Journal of Microbiological Methods |
| Volume | 83 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 Nov 1 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
- Microbiology (medical)
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Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. / Lee, Kyungwon; Kim, Chang Ki; Yong, DongEun; Jeong, Seokhoon; Yum, Jong Hwa; Seo, Young Hee; Docquier, Jean Denis; Chong, Yunsop.
In: Journal of Microbiological Methods, Vol. 83, No. 2, 01.11.2010, p. 149-152.Research output: Contribution to journal › Article
TY - JOUR
T1 - Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli
AU - Lee, Kyungwon
AU - Kim, Chang Ki
AU - Yong, DongEun
AU - Jeong, Seokhoon
AU - Yum, Jong Hwa
AU - Seo, Young Hee
AU - Docquier, Jean Denis
AU - Chong, Yunsop
PY - 2010/11/1
Y1 - 2010/11/1
N2 - The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.
AB - The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.
UR - http://www.scopus.com/inward/record.url?scp=77957874490&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957874490&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2010.08.010
DO - 10.1016/j.mimet.2010.08.010
M3 - Article
C2 - 20801167
AN - SCOPUS:77957874490
VL - 83
SP - 149
EP - 152
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
SN - 0167-7012
IS - 2
ER -