Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Kyungwon Lee; Chang Ki Kim; Dongeun Yong; Seok Hoon Jeong; Jong Hwa Yum; Young Hee Seo; Jean Denis Docquier; Yunsop Chong

doi:10.1016/j.mimet.2010.08.010

Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Kyungwon Lee, Chang Ki Kim, Dongeun Yong, Seok Hoon Jeong, Jong Hwa Yum, Young Hee Seo, Jean Denis Docquier, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article

47 Citations (Scopus)

Abstract

The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.

Original language	English
Pages (from-to)	149-152
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2010.08.010
Publication status	Published - 2010 Nov 1

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

Access to Document

10.1016/j.mimet.2010.08.010

Fingerprint Dive into the research topics of 'Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli'. Together they form a unique fingerprint.

Cite this

Lee, K., Kim, C. K., Yong, D., Jeong, S. H., Yum, J. H., Seo, Y. H., Docquier, J. D., & Chong, Y. (2010). Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. Journal of Microbiological Methods, 83(2), 149-152. https://doi.org/10.1016/j.mimet.2010.08.010

@article{fae5cee782c84240b71a8043b2a38887,

title = "Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli",

abstract = "The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.",

author = "Kyungwon Lee and Kim, {Chang Ki} and Dongeun Yong and Jeong, {Seok Hoon} and Yum, {Jong Hwa} and Seo, {Young Hee} and Docquier, {Jean Denis} and Yunsop Chong",

year = "2010",

month = nov,

day = "1",

doi = "10.1016/j.mimet.2010.08.010",

language = "English",

volume = "83",

pages = "149--152",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "2",

}

Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. / Lee, Kyungwon; Kim, Chang Ki; Yong, Dongeun ; Jeong, Seok Hoon; Yum, Jong Hwa; Seo, Young Hee; Docquier, Jean Denis; Chong, Yunsop.

In: Journal of Microbiological Methods, Vol. 83, No. 2, 01.11.2010, p. 149-152.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

AU - Lee, Kyungwon

AU - Kim, Chang Ki

AU - Yong, Dongeun

AU - Jeong, Seok Hoon

AU - Yum, Jong Hwa

AU - Seo, Young Hee

AU - Docquier, Jean Denis

AU - Chong, Yunsop

PY - 2010/11/1

Y1 - 2010/11/1

N2 - The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.

AB - The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.

UR - http://www.scopus.com/inward/record.url?scp=77957874490&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957874490&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2010.08.010

DO - 10.1016/j.mimet.2010.08.010

M3 - Article

C2 - 20801167

AN - SCOPUS:77957874490

VL - 83

SP - 149

EP - 152

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 2

ER -