TY - JOUR
T1 - Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli
AU - Lee, Kyungwon
AU - Kim, Chang Ki
AU - Yong, Dongeun
AU - Jeong, Seok Hoon
AU - Yum, Jong Hwa
AU - Seo, Young Hee
AU - Docquier, Jean Denis
AU - Chong, Yunsop
PY - 2010/11
Y1 - 2010/11
N2 - The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.
AB - The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.
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U2 - 10.1016/j.mimet.2010.08.010
DO - 10.1016/j.mimet.2010.08.010
M3 - Article
C2 - 20801167
AN - SCOPUS:77957874490
VL - 83
SP - 149
EP - 152
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
SN - 0167-7012
IS - 2
ER -