Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Kyungwon Lee; Chang Ki Kim; Dongeun Yong; Seok Hoon Jeong; Jong Hwa Yum; Young Hee Seo; Jean Denis Docquier; Yunsop Chong

doi:10.1016/j.mimet.2010.08.010

Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Kyungwon Lee, Chang Ki Kim, Dongeun Yong, Seok Hoon Jeong, Jong Hwa Yum, Young Hee Seo, Jean Denis Docquier, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.

Original language	English
Pages (from-to)	149-152
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2010.08.010
Publication status	Published - 2010 Nov

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2010.08.010

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title = "Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli",

abstract = "The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.",

author = "Kyungwon Lee and Kim, {Chang Ki} and Dongeun Yong and Jeong, {Seok Hoon} and Yum, {Jong Hwa} and Seo, {Young Hee} and Docquier, {Jean Denis} and Yunsop Chong",

year = "2010",

month = nov,

doi = "10.1016/j.mimet.2010.08.010",

language = "English",

volume = "83",

pages = "149--152",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "2",

}

Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. / Lee, Kyungwon; Kim, Chang Ki; Yong, Dongeun ; Jeong, Seok Hoon; Yum, Jong Hwa; Seo, Young Hee; Docquier, Jean Denis; Chong, Yunsop.

In: Journal of Microbiological Methods, Vol. 83, No. 2, 11.2010, p. 149-152.

Research output: Contribution to journal › Article › peer-review

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T1 - Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

AU - Lee, Kyungwon

AU - Kim, Chang Ki

AU - Yong, Dongeun

AU - Jeong, Seok Hoon

AU - Yum, Jong Hwa

AU - Seo, Young Hee

AU - Docquier, Jean Denis

AU - Chong, Yunsop

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AB - The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.

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Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Abstract

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