Improved yield and functional parameters of rat pancreas islets isolated under intramuscular anesthesia

Joon Ye Kim, Jeong Ik Lee, Jin Ho Jeong, Yuhui Fang, Man Ki Ju, Soo Jin Kim, Kyu Ha Huh, Myoung Soo Kim, Yu Seun Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Intraperitoneal (IP) anesthesia is commonly used for laboratory animal experiments including rat islet isolation. However, the direct effects of anesthetics on pancreatic islets have been neglected. This study compared the islet function and recovery yield from rats that were anesthetized using IP and intramuscular (IM) injection. In addition, the lag time required to lose deep pain was measured in the following anesthetics combinations. Lewis rats were anesthetized using ketamine and xylazine (K/X) or zoletil and xylazine (Z/X). A glucose challenge test was performed on each group of prepared islets. The effect of the anesthetic agents (e.g., ketamine, zoletil, xylazine alone, and the combination of K/X and Z/X) on cell lines (rat insulinoma; RIN-5F) was investigated by determining their effect on the cell viability, the amount of insulin, and insulin mRNA expression levels of RIN-5F. The time needed for deep anesthesia in IM anesthesia was significantly shortened in comparison to IP [K/X (IM: 313 ± 66 s, IP: 371 ± 84 s) and Z/X (IM: 206 ± 76 s, IP: 245 ± 92 s)]. In addition, number of isolated islet yield by IM anesthesia was significantly improved [K/X (IM: 1530 ± 242, IP: 1245 ± 149) and Z/X (IM: 1136 ± 226, IP: 511 ± 154)]. The functions of fresh islets, indicated by the stimulation index, acquired under IM anesthesia was better preserved than that of IP. The viability and the insulin secretion of RIN-5F were decreased at 24 and 48 h. Insulin gene expression levels were decreased at 24 h as well. Anesthetics may be absorbed through the pancreas surface to the islets and have a direct effect, resulting in islet exposure and deterioration during isolation. In conclusion, for rodent islet isolation, IM anesthesia is simpler and safer in comparison to IP anesthesia.

Original languageEnglish
Pages (from-to)743-750
Number of pages8
JournalCell transplantation
Volume19
Issue number6-7
DOIs
Publication statusPublished - 2010 Nov 1

Fingerprint

Xylazine
Anesthetics
Insulin
Rats
Pancreas
Anesthesia
Ketamine
Cells
Gene expression
Glucose
Deterioration
Animals
Recovery
Insulinoma
Intramuscular Injections
Recovery of Function
Laboratory Animals
Intraperitoneal Injections
Islets of Langerhans
Experiments

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Kim, Joon Ye ; Lee, Jeong Ik ; Jeong, Jin Ho ; Fang, Yuhui ; Ju, Man Ki ; Kim, Soo Jin ; Huh, Kyu Ha ; Kim, Myoung Soo ; Kim, Yu Seun. / Improved yield and functional parameters of rat pancreas islets isolated under intramuscular anesthesia. In: Cell transplantation. 2010 ; Vol. 19, No. 6-7. pp. 743-750.
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abstract = "Intraperitoneal (IP) anesthesia is commonly used for laboratory animal experiments including rat islet isolation. However, the direct effects of anesthetics on pancreatic islets have been neglected. This study compared the islet function and recovery yield from rats that were anesthetized using IP and intramuscular (IM) injection. In addition, the lag time required to lose deep pain was measured in the following anesthetics combinations. Lewis rats were anesthetized using ketamine and xylazine (K/X) or zoletil and xylazine (Z/X). A glucose challenge test was performed on each group of prepared islets. The effect of the anesthetic agents (e.g., ketamine, zoletil, xylazine alone, and the combination of K/X and Z/X) on cell lines (rat insulinoma; RIN-5F) was investigated by determining their effect on the cell viability, the amount of insulin, and insulin mRNA expression levels of RIN-5F. The time needed for deep anesthesia in IM anesthesia was significantly shortened in comparison to IP [K/X (IM: 313 ± 66 s, IP: 371 ± 84 s) and Z/X (IM: 206 ± 76 s, IP: 245 ± 92 s)]. In addition, number of isolated islet yield by IM anesthesia was significantly improved [K/X (IM: 1530 ± 242, IP: 1245 ± 149) and Z/X (IM: 1136 ± 226, IP: 511 ± 154)]. The functions of fresh islets, indicated by the stimulation index, acquired under IM anesthesia was better preserved than that of IP. The viability and the insulin secretion of RIN-5F were decreased at 24 and 48 h. Insulin gene expression levels were decreased at 24 h as well. Anesthetics may be absorbed through the pancreas surface to the islets and have a direct effect, resulting in islet exposure and deterioration during isolation. In conclusion, for rodent islet isolation, IM anesthesia is simpler and safer in comparison to IP anesthesia.",
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Improved yield and functional parameters of rat pancreas islets isolated under intramuscular anesthesia. / Kim, Joon Ye; Lee, Jeong Ik; Jeong, Jin Ho; Fang, Yuhui; Ju, Man Ki; Kim, Soo Jin; Huh, Kyu Ha; Kim, Myoung Soo; Kim, Yu Seun.

In: Cell transplantation, Vol. 19, No. 6-7, 01.11.2010, p. 743-750.

Research output: Contribution to journalArticle

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T1 - Improved yield and functional parameters of rat pancreas islets isolated under intramuscular anesthesia

AU - Kim, Joon Ye

AU - Lee, Jeong Ik

AU - Jeong, Jin Ho

AU - Fang, Yuhui

AU - Ju, Man Ki

AU - Kim, Soo Jin

AU - Huh, Kyu Ha

AU - Kim, Myoung Soo

AU - Kim, Yu Seun

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AB - Intraperitoneal (IP) anesthesia is commonly used for laboratory animal experiments including rat islet isolation. However, the direct effects of anesthetics on pancreatic islets have been neglected. This study compared the islet function and recovery yield from rats that were anesthetized using IP and intramuscular (IM) injection. In addition, the lag time required to lose deep pain was measured in the following anesthetics combinations. Lewis rats were anesthetized using ketamine and xylazine (K/X) or zoletil and xylazine (Z/X). A glucose challenge test was performed on each group of prepared islets. The effect of the anesthetic agents (e.g., ketamine, zoletil, xylazine alone, and the combination of K/X and Z/X) on cell lines (rat insulinoma; RIN-5F) was investigated by determining their effect on the cell viability, the amount of insulin, and insulin mRNA expression levels of RIN-5F. The time needed for deep anesthesia in IM anesthesia was significantly shortened in comparison to IP [K/X (IM: 313 ± 66 s, IP: 371 ± 84 s) and Z/X (IM: 206 ± 76 s, IP: 245 ± 92 s)]. In addition, number of isolated islet yield by IM anesthesia was significantly improved [K/X (IM: 1530 ± 242, IP: 1245 ± 149) and Z/X (IM: 1136 ± 226, IP: 511 ± 154)]. The functions of fresh islets, indicated by the stimulation index, acquired under IM anesthesia was better preserved than that of IP. The viability and the insulin secretion of RIN-5F were decreased at 24 and 48 h. Insulin gene expression levels were decreased at 24 h as well. Anesthetics may be absorbed through the pancreas surface to the islets and have a direct effect, resulting in islet exposure and deterioration during isolation. In conclusion, for rodent islet isolation, IM anesthesia is simpler and safer in comparison to IP anesthesia.

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