Improvement in H2 production from Clostridium butyricum by co-culture with Sporolactobacillus vineae

Jong Hun Park, Do Hyung Kim, Jong Hyun Baik, Jeong Hoon Park, Jeong Joon Yoon, Chae Young Lee, Sang Hyoun Kim

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14 Citations (Scopus)


Dark fermentation has emerged as a promising method for converting waste into biohydrogen, a clean and sustainable energy source. However, the interaction between biohydrogen production performance and its microbial community has not been investigated sufficiently. This study investigated the effect of S. vineae on hydrogen production using defined cultures. Co-cultures of Clostridium butyricum (C. butyricum) with Sporolactobacillus vineae (S. vineae) increased the hydrogen production from 1.57 to 1.84 mol H2/mol glucoseadded, whereas lactic acid production did not increase in comparison with single culture of C. butyricum. At all the examined co-culture conditions, C. butyricum occupied more than 90% of the microbial composition; therefore, the microbial community analysis alone could not explain the difference in hydrogen production according to S. vineae addition. PICRUSt analysis showed that co-culture with S. vineae enhanced the expression of genes relating lactate to butyrate and H2 pathway, although S. vineae did not produce butyrate. Co-cultures of Clostridium sp. and Sporolactobacillus sp. could be beneficial for H2 production from the viewpoint of acidogenic pathway regulation. This study is expected to contribute to the understanding of the synergistic mechanisms of both the strains to obtain optimal biohydrogen production.

Original languageEnglish
Article number119051
Publication statusPublished - 2021 Feb 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT, MSIT) ( NRF-2019M3E6A1103839 , NRF-2020R1A2B5B02001757 ).

Publisher Copyright:
© 2020 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Fuel Technology
  • Energy Engineering and Power Technology
  • Organic Chemistry


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