Improvement of hydrogen fermentation of galactose by combined inoculation strategy

Periyasamy Sivagurunathan, Parthiban Anburajan, Gopalakrishnan Kumar, Pugazhendhi Arivalagan, Péter Bakonyi, Sang Hyoun Kim

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


This study evaluated the feasibility of anaerobic hydrogen fermentation of galactose, a red algal biomass sugar, using individual and combined mixed culture inocula. Heat-treated (90°C, 30 min) samples of granular sludge (GS) and suspended digester sludge (SDS) were used as inoculum sources. The type of mixed culture inoculum played an important role in hydrogen production from galactose. Between two inocula, granular sludge showed higher hydrogen production rate (HPR) and hydrogen yield (HY) of 2.2 L H2/L-d and 1.09 mol H2/mol galactoseadded, respectively. Combined inoculation (GS + SDS) led to an elevated HPR and HY of 3.1 L H2/L-d and 1.28 mol H2/mol galactoseadded, respectively. Acetic and butyric acids are the major organic acids during fermentation. Quantitative polymerase chain reaction (qPCR) revealed that the mixed culture generated using the combined inoculation contained a higher cluster I Clostridium abundance than the culture produced using the single inoculum.

Original languageEnglish
Pages (from-to)353-357
Number of pages5
JournalJournal of Bioscience and Bioengineering
Issue number3
Publication statusPublished - 2017 Mar 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 2014R1A2A2A04005475).

Publisher Copyright:
© 2016 The Society for Biotechnology, Japan

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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