Improvement of in vitro two-stage transformation assay and determination of the promotional effect of cadmium

Ming Zhu Fang, Dae Yong Kim, Han Woong Lee, Myung Haing Cho

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The classical in vitro two-stage transformation method was modified for high transformation frequency, and the promotional effect of cadmium was evaluated. In this study, we reconfirmed the usefulness of the replating method and the optimal duration time between the initiator and promoter treatments for the optimal transformation of the Balb/3T3 cells. The results also showed that subsequent exposure to CdCl2 for 2 weeks after initial exposure to N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) markedly enhanced the transformation frequency. At the concentration of 360 ng/ml, the transformation frequency was increased by 35-fold that of the cultures treated only with MNNG, and was higher than that of the positive control group treated with 100 ng/ml 12-O-tetradecanoyl phorbol-13-acetate (TPA) after MNNG treatment. This transformation frequency was higher than that reported previously. Therefore, this in vitro two-stage transformation method can be used efficiently for the screening of genotoxic and non-genotoxic carcinogens and the study of multistage carcinogenesis. These results also indicate that cadmium has a strong potency as a promoter, and the promotional effect of cadmium is higher than that of TPA.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalToxicology in Vitro
Volume15
Issue number3
DOIs
Publication statusPublished - 2001 Jun 6

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Cadmium
Nitrosoguanidines
Assays
Methylnitronitrosoguanidine
Cadmium Chloride
3T3 Cells
Tetradecanoylphorbol Acetate
Carcinogens
Screening
Carcinogenesis
Acetates
Control Groups
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

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abstract = "The classical in vitro two-stage transformation method was modified for high transformation frequency, and the promotional effect of cadmium was evaluated. In this study, we reconfirmed the usefulness of the replating method and the optimal duration time between the initiator and promoter treatments for the optimal transformation of the Balb/3T3 cells. The results also showed that subsequent exposure to CdCl2 for 2 weeks after initial exposure to N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) markedly enhanced the transformation frequency. At the concentration of 360 ng/ml, the transformation frequency was increased by 35-fold that of the cultures treated only with MNNG, and was higher than that of the positive control group treated with 100 ng/ml 12-O-tetradecanoyl phorbol-13-acetate (TPA) after MNNG treatment. This transformation frequency was higher than that reported previously. Therefore, this in vitro two-stage transformation method can be used efficiently for the screening of genotoxic and non-genotoxic carcinogens and the study of multistage carcinogenesis. These results also indicate that cadmium has a strong potency as a promoter, and the promotional effect of cadmium is higher than that of TPA.",
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Improvement of in vitro two-stage transformation assay and determination of the promotional effect of cadmium. / Fang, Ming Zhu; Kim, Dae Yong; Lee, Han Woong; Cho, Myung Haing.

In: Toxicology in Vitro, Vol. 15, No. 3, 06.06.2001, p. 225-231.

Research output: Contribution to journalArticle

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