Improvement of lipoprotein separation with a guard channel prior to asymmetrical flow field-flow fractionation using fluorescence detection

Ju Yong Lee, Donghoon Choi, Christoph Johan, Myeong Hee Moon

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In this article, a simple experimental approach to improve lipoprotein separation and detection in flow field-flow fractionation (FlFFF) is detailed. Lipoproteins are globular particles composed of lipids and proteins in blood serum and their roles include transferring fats and cholesterols through blood vessels throughout the body. Especially, presence of small, dense low-density lipoproteins (LDL) is associated with cardiovascular risk. Two experimental approaches were explored in this study: an increase in the reproducibility of LDL particle separation by implementing a guard channel prior to an asymmetrical FlFFF (AFlFFF) channel in order to deplete small molecular weight serum proteins and reducing the required injection volume of a serum sample by implementing fluorescence detection. The guard channel was made of a simple hollow fiber module so that the serum sample can be washed with the help of radial flow prior to injection into the AFlFFF channel. The channel was tested with protein standards and serum samples to ensure precision of the retention time and the protein recovery rate. A fluorescent phospholipid dye was utilized to label lipoprotein particles before separation for fluorescence detection, which resulted in a reduction of the required injection volume of serum.

Original languageEnglish
Pages (from-to)4144-4148
Number of pages5
JournalJournal of Chromatography A
Volume1218
Issue number27
DOIs
Publication statusPublished - 2011 Jul 8

Fingerprint

Field Flow Fractionation
Fractionation
Lipoproteins
Flow fields
Fluorescence
LDL Lipoproteins
Serum
Injections
Blood Proteins
Radial flow
Proteins
Blood vessels
Labels
Phospholipids
Blood
Coloring Agents
Molecular weight
Fats
Fluorescent Dyes
Cholesterol

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

@article{5beab39ed61c47f394d98f0c986a95f0,
title = "Improvement of lipoprotein separation with a guard channel prior to asymmetrical flow field-flow fractionation using fluorescence detection",
abstract = "In this article, a simple experimental approach to improve lipoprotein separation and detection in flow field-flow fractionation (FlFFF) is detailed. Lipoproteins are globular particles composed of lipids and proteins in blood serum and their roles include transferring fats and cholesterols through blood vessels throughout the body. Especially, presence of small, dense low-density lipoproteins (LDL) is associated with cardiovascular risk. Two experimental approaches were explored in this study: an increase in the reproducibility of LDL particle separation by implementing a guard channel prior to an asymmetrical FlFFF (AFlFFF) channel in order to deplete small molecular weight serum proteins and reducing the required injection volume of a serum sample by implementing fluorescence detection. The guard channel was made of a simple hollow fiber module so that the serum sample can be washed with the help of radial flow prior to injection into the AFlFFF channel. The channel was tested with protein standards and serum samples to ensure precision of the retention time and the protein recovery rate. A fluorescent phospholipid dye was utilized to label lipoprotein particles before separation for fluorescence detection, which resulted in a reduction of the required injection volume of serum.",
author = "Lee, {Ju Yong} and Donghoon Choi and Christoph Johan and Moon, {Myeong Hee}",
year = "2011",
month = "7",
day = "8",
doi = "10.1016/j.chroma.2010.11.079",
language = "English",
volume = "1218",
pages = "4144--4148",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "27",

}

Improvement of lipoprotein separation with a guard channel prior to asymmetrical flow field-flow fractionation using fluorescence detection. / Lee, Ju Yong; Choi, Donghoon; Johan, Christoph; Moon, Myeong Hee.

In: Journal of Chromatography A, Vol. 1218, No. 27, 08.07.2011, p. 4144-4148.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Improvement of lipoprotein separation with a guard channel prior to asymmetrical flow field-flow fractionation using fluorescence detection

AU - Lee, Ju Yong

AU - Choi, Donghoon

AU - Johan, Christoph

AU - Moon, Myeong Hee

PY - 2011/7/8

Y1 - 2011/7/8

N2 - In this article, a simple experimental approach to improve lipoprotein separation and detection in flow field-flow fractionation (FlFFF) is detailed. Lipoproteins are globular particles composed of lipids and proteins in blood serum and their roles include transferring fats and cholesterols through blood vessels throughout the body. Especially, presence of small, dense low-density lipoproteins (LDL) is associated with cardiovascular risk. Two experimental approaches were explored in this study: an increase in the reproducibility of LDL particle separation by implementing a guard channel prior to an asymmetrical FlFFF (AFlFFF) channel in order to deplete small molecular weight serum proteins and reducing the required injection volume of a serum sample by implementing fluorescence detection. The guard channel was made of a simple hollow fiber module so that the serum sample can be washed with the help of radial flow prior to injection into the AFlFFF channel. The channel was tested with protein standards and serum samples to ensure precision of the retention time and the protein recovery rate. A fluorescent phospholipid dye was utilized to label lipoprotein particles before separation for fluorescence detection, which resulted in a reduction of the required injection volume of serum.

AB - In this article, a simple experimental approach to improve lipoprotein separation and detection in flow field-flow fractionation (FlFFF) is detailed. Lipoproteins are globular particles composed of lipids and proteins in blood serum and their roles include transferring fats and cholesterols through blood vessels throughout the body. Especially, presence of small, dense low-density lipoproteins (LDL) is associated with cardiovascular risk. Two experimental approaches were explored in this study: an increase in the reproducibility of LDL particle separation by implementing a guard channel prior to an asymmetrical FlFFF (AFlFFF) channel in order to deplete small molecular weight serum proteins and reducing the required injection volume of a serum sample by implementing fluorescence detection. The guard channel was made of a simple hollow fiber module so that the serum sample can be washed with the help of radial flow prior to injection into the AFlFFF channel. The channel was tested with protein standards and serum samples to ensure precision of the retention time and the protein recovery rate. A fluorescent phospholipid dye was utilized to label lipoprotein particles before separation for fluorescence detection, which resulted in a reduction of the required injection volume of serum.

UR - http://www.scopus.com/inward/record.url?scp=79958810235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958810235&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2010.11.079

DO - 10.1016/j.chroma.2010.11.079

M3 - Article

VL - 1218

SP - 4144

EP - 4148

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 27

ER -