In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring

Hyeong Rae Kim, Sanggwon An, Jungho Hwang, Jae Hong Park, Jeong Hoon Byeon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Simultaneous improvement in detection speed and reliability is critical for bioaerosol monitoring. Recent rapid detection strategies exhibit difficulties with misinterpretation due to signal interference from co-existing nonbiological particles, whereas biomolecular and bioluminescent approaches require long process times (>several tens of minutes) to generate readable values despite their better detection reliability. To overcome these shortcomings, we designed a system to achieve rapid reliable field detection of bioaerosols (>104 relative luminescence units [RLU] per cubic meter of air) in <3 min processing time (equivalent to 24 L sampling air volume) by employing a lysis droplet supply for efficient extraction of adenosine triphosphate (ATP) from particulate matter (PM) and a photomultiplier tube detector for signal amplification of ATP bioluminescence. We also suggested the use of the ratio of RLU (m−3) to total PM (μg m−3), or specific bioluminescence (RLU μg−1), as a measure of the biofraction of PM (i.e., potential biohazards). A correlation between RLU and colony forming unit was also obtained from simultaneous aerosol sampling using an agar-inserted sampler.

Original languageEnglish
Pages (from-to)684-690
Number of pages7
JournalJournal of Hazardous Materials
Volume369
DOIs
Publication statusPublished - 2019 May 5

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

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