In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring

Hyeong Rae Kim, Sanggwon An, Jungho Hwang, Jae Hong Park, Jeong Hoon Byeon

Research output: Contribution to journalArticle

Abstract

Simultaneous improvement in detection speed and reliability is critical for bioaerosol monitoring. Recent rapid detection strategies exhibit difficulties with misinterpretation due to signal interference from co-existing nonbiological particles, whereas biomolecular and bioluminescent approaches require long process times (>several tens of minutes) to generate readable values despite their better detection reliability. To overcome these shortcomings, we designed a system to achieve rapid reliable field detection of bioaerosols (>104 relative luminescence units [RLU] per cubic meter of air) in <3 min processing time (equivalent to 24 L sampling air volume) by employing a lysis droplet supply for efficient extraction of adenosine triphosphate (ATP) from particulate matter (PM) and a photomultiplier tube detector for signal amplification of ATP bioluminescence. We also suggested the use of the ratio of RLU (m−3) to total PM (μg m−3), or specific bioluminescence (RLU μg−1), as a measure of the biofraction of PM (i.e., potential biohazards). A correlation between RLU and colony forming unit was also obtained from simultaneous aerosol sampling using an agar-inserted sampler.

Original languageEnglish
Pages (from-to)684-690
Number of pages7
JournalJournal of Hazardous Materials
Volume369
DOIs
Publication statusPublished - 2019 May 5

Fingerprint

lysis
luminescence
Luminescence
Dust
Particles (particulate matter)
droplet
Particulate Matter
Adenosine Triphosphate
Bioluminescence
dust
particulate matter
bioluminescence
Monitoring
monitoring
Biohazards
Air
Sampling
Hazardous Substances
air sampling
Photomultipliers

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Kim, Hyeong Rae ; An, Sanggwon ; Hwang, Jungho ; Park, Jae Hong ; Byeon, Jeong Hoon. / In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring. In: Journal of Hazardous Materials. 2019 ; Vol. 369. pp. 684-690.
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In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring. / Kim, Hyeong Rae; An, Sanggwon; Hwang, Jungho; Park, Jae Hong; Byeon, Jeong Hoon.

In: Journal of Hazardous Materials, Vol. 369, 05.05.2019, p. 684-690.

Research output: Contribution to journalArticle

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