In Situ Recruitment of Human Bone Marrow-Derived Mesenchymal Stem Cells Using Chemokines for Articular Cartilage Regeneration

Min Sung Park, Yun Hee Kim, Youngmee Jung, Soo Hyun Kim, Jong Chul Park, Dong Suk Yoon, Sung Hwan Kim, Jin Woo Lee

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are a good cell source for regeneration of cartilage as they can migrate directly to the site of cartilage injury and differentiate into articular chondrocytes. Articular cartilage defects do not heal completely due to the lack of chondrocytes or BMSCs at the site of injury. In this study, the chemotaxis of BMSCs toward chemokines, which may give rise to a complete regeneration of the articular cartilage, was investigated. CCR2, CCR4, CCR6, CXCR1, and CXCR2 were expressed in normal BMSCs and were increased significantly upon treatment with proinflammatory cytokines. BMSC migration was increased by MIP-3a and IL-8 more than by MCP-1 or SDF-1a. IL-8 and MIP-3a significantly enhanced the chemotaxis of BMSCs compared with MCP-1, SDF-1a, or PBS. Human BMSC recruitment to transplanted scaffolds containing either IL-8 or MIP-3a significantly increased in vivo compared to scaffolds containing PBS. Furthermore, IL-8-and MIP-3a-containing scaffolds enhanced tissue regeneration of an osteochondral defect site in beagle knee articular cartilage. Therefore, this study suggests that IL-8 and MIP-3a are the candidates that induce the regeneration of damaged articular cartilage.

Original languageEnglish
Pages (from-to)1067-1083
Number of pages17
JournalCell transplantation
Volume24
Issue number6
DOIs
Publication statusPublished - 2015

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS: This work was supported by a faculty research grant of Yonsei University College of Medicine (6-2009-0153) and by a grant (Code: A110328) from the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea. The authors declare no conflicts of interest.

Publisher Copyright:
© 2015 Cognizant Comm. Corp.

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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