In vitro activity of tigecycline alone and antimicrobial combinations against clinical Neisseria gonorrhoeae isolates

Hyukmin Lee, Hyunsoo Kim, Young Hee Seo, DongEun Yong, Seokhoon Jeong, Kyungwon Lee, Younsop Chong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study, we determined the in vitro activity of various combinations of antimicrobial agents against 54 Neisseria gonorrhoeae isolates. The combined activity of ceftriaxone (CRO) and azithromycin (AZM), CRO and doxycycline (DOX), CRO and spectinomycin (SPT), cefixime (CFX) and AZM, CFX and DOX, and CFX and SPT was determined using a checkerboard method. The fractional inhibitory concentration index (FICI) values for all combinations were either additive or indifferent, and no synergistic or antagonistic effects were found. The FICI comparison in each combination did not show any difference according to the N.gonorrhoeae-resistant phenotypes and genotypic characteristics, including penicillinase-producing N. gonorrhoeae, tetracycline-resistant N. gonorrhoeae, stratified MIC of all antibiotics, and N. gonorrhoeae multiantigen sequence typing. MIC50 and MIC90 of tigecycline by agar dilution were 0.5 mg/L and 0.5 mg/L, respectively, which were lower than that of tetracycline and DOX. Additive/indifference results could suggest that combinations that include CRO may be used safely without a significant likelihood of generating resistance.

Original languageEnglish
Pages (from-to)160-162
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume87
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

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Ceftriaxone
Neisseria gonorrhoeae
Cefixime
Doxycycline
Spectinomycin
Azithromycin
Tetracycline
Penicillinase
Gonorrhea
Anti-Infective Agents
Agar
Anti-Bacterial Agents
Phenotype
tigecycline
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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abstract = "In this study, we determined the in vitro activity of various combinations of antimicrobial agents against 54 Neisseria gonorrhoeae isolates. The combined activity of ceftriaxone (CRO) and azithromycin (AZM), CRO and doxycycline (DOX), CRO and spectinomycin (SPT), cefixime (CFX) and AZM, CFX and DOX, and CFX and SPT was determined using a checkerboard method. The fractional inhibitory concentration index (FICI) values for all combinations were either additive or indifferent, and no synergistic or antagonistic effects were found. The FICI comparison in each combination did not show any difference according to the N.gonorrhoeae-resistant phenotypes and genotypic characteristics, including penicillinase-producing N. gonorrhoeae, tetracycline-resistant N. gonorrhoeae, stratified MIC of all antibiotics, and N. gonorrhoeae multiantigen sequence typing. MIC50 and MIC90 of tigecycline by agar dilution were 0.5 mg/L and 0.5 mg/L, respectively, which were lower than that of tetracycline and DOX. Additive/indifference results could suggest that combinations that include CRO may be used safely without a significant likelihood of generating resistance.",
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In vitro activity of tigecycline alone and antimicrobial combinations against clinical Neisseria gonorrhoeae isolates. / Lee, Hyukmin; Kim, Hyunsoo; Seo, Young Hee; Yong, DongEun; Jeong, Seokhoon; Lee, Kyungwon; Chong, Younsop.

In: Diagnostic Microbiology and Infectious Disease, Vol. 87, No. 2, 01.02.2017, p. 160-162.

Research output: Contribution to journalArticle

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