In vitro analysis of nonthermal plasma as a disinfecting agent

Ashlee E. Watts, Susan L. Fubini, Margaret Vernier-Singer, Czeslaw Golkowski, SungJae Shin, Rory J. Todhunter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective - To determine the effect of nonthermal plasma on Staphylococcus aureus, fibroblasts in monolayer culture, and clean and contaminated skin explants. Sample Population - Normal skin from euthanized horses. Procedures - S aureus organisms were plated and treated with nonthermal plasma followed by bacterial culture to assess viability. Fibroblasts in monolayer culture and the epidermal and dermal surfaces of clean and S aureus-contaminated skin explants were treated. The effects of distance and duration on the response to treatment were compared. Results - Compared with controls, treatment with nonthermal plasma resulted in significantly decreased bacterial growth and significantly inhibited survival of fibroblasts in monolayer culture. When epidermal and dermal surfaces of skin explants were treated, there was no effect on production of normal fibroblasts during explant culture, except when extended exposure times of ≥ 2 minutes were used. Treatment with nonthermal plasma resulted in significantly lower bacterial counts after 24 hours of culture of S aureus-contaminated epidermis but not of dermis. Conclusions and Clinical Relevance - Nonthermal plasma resulted in bacterial decontamination of agar and epithelium; negative effects on fibroblasts in monolayer; and no negative effects on skin explants, except at long exposure times. Use of nonthermal plasma appears safe for treatment of epithelialized surfaces, may be safe for granulating wounds, and results in decontamination of S aureus. Investigations on the effects that nonthermal plasma may have on patient tissues pre indicated with a clinically applicable delivery device.

Original languageEnglish
Pages (from-to)2030-2035
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume67
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

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disinfectants
skin (animal)
fibroblasts
explants
Skin
Fibroblasts
decontamination
exposure duration
Decontamination
dermis
epidermis (animal)
animal injuries
plate count
microbial growth
Staphylococcus aureus
Bacterial Load
epithelium
agar
Therapeutics
Dermis

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Watts, A. E., Fubini, S. L., Vernier-Singer, M., Golkowski, C., Shin, S., & Todhunter, R. J. (2006). In vitro analysis of nonthermal plasma as a disinfecting agent. American Journal of Veterinary Research, 67(12), 2030-2035. https://doi.org/10.2460/ajvr.67.12.2030
Watts, Ashlee E. ; Fubini, Susan L. ; Vernier-Singer, Margaret ; Golkowski, Czeslaw ; Shin, SungJae ; Todhunter, Rory J. / In vitro analysis of nonthermal plasma as a disinfecting agent. In: American Journal of Veterinary Research. 2006 ; Vol. 67, No. 12. pp. 2030-2035.
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Watts, AE, Fubini, SL, Vernier-Singer, M, Golkowski, C, Shin, S & Todhunter, RJ 2006, 'In vitro analysis of nonthermal plasma as a disinfecting agent', American Journal of Veterinary Research, vol. 67, no. 12, pp. 2030-2035. https://doi.org/10.2460/ajvr.67.12.2030

In vitro analysis of nonthermal plasma as a disinfecting agent. / Watts, Ashlee E.; Fubini, Susan L.; Vernier-Singer, Margaret; Golkowski, Czeslaw; Shin, SungJae; Todhunter, Rory J.

In: American Journal of Veterinary Research, Vol. 67, No. 12, 01.12.2006, p. 2030-2035.

Research output: Contribution to journalArticle

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T1 - In vitro analysis of nonthermal plasma as a disinfecting agent

AU - Watts, Ashlee E.

AU - Fubini, Susan L.

AU - Vernier-Singer, Margaret

AU - Golkowski, Czeslaw

AU - Shin, SungJae

AU - Todhunter, Rory J.

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Y1 - 2006/12/1

N2 - Objective - To determine the effect of nonthermal plasma on Staphylococcus aureus, fibroblasts in monolayer culture, and clean and contaminated skin explants. Sample Population - Normal skin from euthanized horses. Procedures - S aureus organisms were plated and treated with nonthermal plasma followed by bacterial culture to assess viability. Fibroblasts in monolayer culture and the epidermal and dermal surfaces of clean and S aureus-contaminated skin explants were treated. The effects of distance and duration on the response to treatment were compared. Results - Compared with controls, treatment with nonthermal plasma resulted in significantly decreased bacterial growth and significantly inhibited survival of fibroblasts in monolayer culture. When epidermal and dermal surfaces of skin explants were treated, there was no effect on production of normal fibroblasts during explant culture, except when extended exposure times of ≥ 2 minutes were used. Treatment with nonthermal plasma resulted in significantly lower bacterial counts after 24 hours of culture of S aureus-contaminated epidermis but not of dermis. Conclusions and Clinical Relevance - Nonthermal plasma resulted in bacterial decontamination of agar and epithelium; negative effects on fibroblasts in monolayer; and no negative effects on skin explants, except at long exposure times. Use of nonthermal plasma appears safe for treatment of epithelialized surfaces, may be safe for granulating wounds, and results in decontamination of S aureus. Investigations on the effects that nonthermal plasma may have on patient tissues pre indicated with a clinically applicable delivery device.

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Watts AE, Fubini SL, Vernier-Singer M, Golkowski C, Shin S, Todhunter RJ. In vitro analysis of nonthermal plasma as a disinfecting agent. American Journal of Veterinary Research. 2006 Dec 1;67(12):2030-2035. https://doi.org/10.2460/ajvr.67.12.2030