In vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth

JeSeon Song, Seong Oh Kim, Seung Hye Kim, Hyung Jun Choi, Heung Kyu Son, Hansung Jung, ChangSung Kim, Jae Ho Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium.

Original languageEnglish
Pages (from-to)2040-2051
Number of pages12
JournalTissue Engineering - Part A
Volume18
Issue number19-20
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Periodontal Ligament
Deciduous Tooth
Ligaments
Stem cells
Stem Cells
Dental Cementum
Transplants
Polymerase chain reaction
Transcription
Staining and Labeling
Reverse Transcription
Tooth
Periodontium
Polymerase Chain Reaction
Adult Stem Cells
Collagen
Cell Cycle
Genes
Transplantation
Cells

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Song, JeSeon ; Kim, Seong Oh ; Kim, Seung Hye ; Choi, Hyung Jun ; Son, Heung Kyu ; Jung, Hansung ; Kim, ChangSung ; Lee, Jae Ho. / In vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth. In: Tissue Engineering - Part A. 2012 ; Vol. 18, No. 19-20. pp. 2040-2051.
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In vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth. / Song, JeSeon; Kim, Seong Oh; Kim, Seung Hye; Choi, Hyung Jun; Son, Heung Kyu; Jung, Hansung; Kim, ChangSung; Lee, Jae Ho.

In: Tissue Engineering - Part A, Vol. 18, No. 19-20, 01.10.2012, p. 2040-2051.

Research output: Contribution to journalArticle

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AU - Jung, Hansung

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AU - Lee, Jae Ho

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N2 - In many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium.

AB - In many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium.

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