In vitro anti-biofilm activity of macelignan isolated from Myristica fragrans Houtt. against oral primary colonizer bacteria

Yanti, Yaya Rukayadi, Kyu Hoi Kim, Jae-Kwan Hwang

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

In early dental plaque formation, oral primary colonizers such as Streptococcus mutans, Streptococcus sanguis and Actinomyces viscosus are initially attached to the pellicle-coated tooth surface to form a biofilm. The study aimed to determine the efficacy of macelignan, isolated from nutmeg (Myristica fragrans Houtt.), in removing each single oral primary biofilm in vitro on a polystyrene 96-well microtiter plate. Four biofilm growth phases (4, 12, 20 and 24 h) were evaluated in this study after treatment with macelignan at various concentrations (0.2, 2 and 10 μg/mL) and exposure times (5, 10 and 30 min). Anti-biofilm activity of macelignan was measured as the percentage of the remaining biofilm absorbance after macelignan treatment in comparison with the untreated control. At 24 h of biofilm growth, S. mutans, A. viscosus and S. sanguis biofilms were reduced by up to 30%, 30% and 38%, respectively, after treatment with 10 μg/mL macelignan for 5 min. Increasing the treatment time to 30 min resulted in a reduction of more than 50% of each of the single primary biofilms. The results indicate that macelignan is a potent natural anti-biofilm agent against oral primary colonizers.

Original languageEnglish
Pages (from-to)308-312
Number of pages5
JournalPhytotherapy Research
Volume22
Issue number3
DOIs
Publication statusPublished - 2008 Mar 1

Fingerprint

Myristica fragrans
Biofilms
Bacteria
Actinomyces viscosus
Streptococcus sanguis
Streptococcus mutans
macelignan
In Vitro Techniques
Dental Plaque
Polystyrenes
Growth
Tooth

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

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title = "In vitro anti-biofilm activity of macelignan isolated from Myristica fragrans Houtt. against oral primary colonizer bacteria",
abstract = "In early dental plaque formation, oral primary colonizers such as Streptococcus mutans, Streptococcus sanguis and Actinomyces viscosus are initially attached to the pellicle-coated tooth surface to form a biofilm. The study aimed to determine the efficacy of macelignan, isolated from nutmeg (Myristica fragrans Houtt.), in removing each single oral primary biofilm in vitro on a polystyrene 96-well microtiter plate. Four biofilm growth phases (4, 12, 20 and 24 h) were evaluated in this study after treatment with macelignan at various concentrations (0.2, 2 and 10 μg/mL) and exposure times (5, 10 and 30 min). Anti-biofilm activity of macelignan was measured as the percentage of the remaining biofilm absorbance after macelignan treatment in comparison with the untreated control. At 24 h of biofilm growth, S. mutans, A. viscosus and S. sanguis biofilms were reduced by up to 30{\%}, 30{\%} and 38{\%}, respectively, after treatment with 10 μg/mL macelignan for 5 min. Increasing the treatment time to 30 min resulted in a reduction of more than 50{\%} of each of the single primary biofilms. The results indicate that macelignan is a potent natural anti-biofilm agent against oral primary colonizers.",
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In vitro anti-biofilm activity of macelignan isolated from Myristica fragrans Houtt. against oral primary colonizer bacteria. / Yanti; Rukayadi, Yaya; Kim, Kyu Hoi; Hwang, Jae-Kwan.

In: Phytotherapy Research, Vol. 22, No. 3, 01.03.2008, p. 308-312.

Research output: Contribution to journalArticle

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