In Vitro Anti-Inflammatory Activity of Panduratin A Isolated from Kaempferia pandurata in RAW264.7 Cells

Jung Mi Yun, Hoonjeong Kwon, Jae Kwan Hwang

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

An active compound identified as panduratin A was isolated from a methanol extract of Kaempferia pandurata (Zingiberaceae). We examined the effect of panduratin A on nitric oxide (NO) and prostaglandin E2 (PGE 2) production induced by lipopolysaccharide (LPS) in RAW264.7 cells. Modulations of iNOS and COX-2 enzyme expression were evaluated by Western blotting, Panduratin A strongly inhibited both NO (IC50:0.175 μM) and PGE2 (IC50:0.0195 μM) production and suppressed both iNOS and COX-2 enzyme expression without any appreciable cytotoxic effect on RAW264.7 cells in a dose-dependent manner. Panduratin A also suppressed the phosphorylation of inhibitor κBa (IκBα) and degradation of IκBα associated with nuclear factor κB (NF-κB) activation. Furthermore, panduratin A inhibited LPS-induced NF-κB transcriptional activity in a dose-dependent manner. These results suggest that panduratin A could exert its inhibitory effects on the production of NO and PGE2 through the suppression of NF-κB activation, indicating its potential for use as an anti-inflammatory agent.

Original languageEnglish
Pages (from-to)1102-1108
Number of pages7
JournalPlanta Medica
Volume69
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

Fingerprint

Zingiberaceae
Anti-Inflammatory Agents
Nitric Oxide
Corrosion inhibitors
Dinoprostone
Inhibitory Concentration 50
Lipopolysaccharides
Chemical activation
Phosphorylation
Enzymes
Methanol
In Vitro Techniques
panduratin A
Western Blotting
Modulation
Degradation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

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abstract = "An active compound identified as panduratin A was isolated from a methanol extract of Kaempferia pandurata (Zingiberaceae). We examined the effect of panduratin A on nitric oxide (NO) and prostaglandin E2 (PGE 2) production induced by lipopolysaccharide (LPS) in RAW264.7 cells. Modulations of iNOS and COX-2 enzyme expression were evaluated by Western blotting, Panduratin A strongly inhibited both NO (IC50:0.175 μM) and PGE2 (IC50:0.0195 μM) production and suppressed both iNOS and COX-2 enzyme expression without any appreciable cytotoxic effect on RAW264.7 cells in a dose-dependent manner. Panduratin A also suppressed the phosphorylation of inhibitor κBa (IκBα) and degradation of IκBα associated with nuclear factor κB (NF-κB) activation. Furthermore, panduratin A inhibited LPS-induced NF-κB transcriptional activity in a dose-dependent manner. These results suggest that panduratin A could exert its inhibitory effects on the production of NO and PGE2 through the suppression of NF-κB activation, indicating its potential for use as an anti-inflammatory agent.",
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In Vitro Anti-Inflammatory Activity of Panduratin A Isolated from Kaempferia pandurata in RAW264.7 Cells. / Yun, Jung Mi; Kwon, Hoonjeong; Hwang, Jae Kwan.

In: Planta Medica, Vol. 69, No. 12, 01.12.2003, p. 1102-1108.

Research output: Contribution to journalArticle

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