In vitro anti-Malassezia activity of xanthorrhizol isolated from Curcuma xanthorrhiza Roxb

Y. Rukayadi, J. K. Hwang

Research output: Contribution to journalArticle

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Abstract

Aims: This study aimed at investigating the anti-Malassezia activity of xanthorrhizol (XTZ) isolated from Curcuma xanthorrhiza Roxb. against Malassezia furfur ATCC 14521 and Malassezia pachydermatis ATCC 14522. Methods and Results: The in vitro susceptibility tests for XTZ were carried out in terms of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), using broth microdilution method with endpoint after 48 h. Time-kill curves were determined at concentrations ranging from 0 to 25 μg ml-1. The MIC values of XTZ against M. furfur and M. pachydermatis were 1.25 and 0.25 μg ml-1, respectively. The MFC of XTZ was 5 μg ml-1 for M. furfur and 2.5 μg ml-1 for M. pachydermatis. Time-kill curves demonstrated that treatment with 25 μg ml-1 of XTZ for 5 h was able to kill 100% of M. furfur, while 20 μg ml-1 of XTZ for 15 min killed M. pachydermatis completely. Conclusion: XTZ shows potential as an anti-Malassezia agent for inhibiting the growth of M. furfur ATCC 14521 and M. pachydermatis ATCC 14522 in vitro. Significance and Impact of the Study: XTZ may be a useful alternative for treating Malassezia-associated diseases.

Original languageEnglish
Pages (from-to)126-130
Number of pages5
JournalLetters in Applied Microbiology
Volume44
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

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Malassezia
Curcuma
Microbial Sensitivity Tests
In Vitro Techniques
xanthorrhizol

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology

Cite this

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title = "In vitro anti-Malassezia activity of xanthorrhizol isolated from Curcuma xanthorrhiza Roxb",
abstract = "Aims: This study aimed at investigating the anti-Malassezia activity of xanthorrhizol (XTZ) isolated from Curcuma xanthorrhiza Roxb. against Malassezia furfur ATCC 14521 and Malassezia pachydermatis ATCC 14522. Methods and Results: The in vitro susceptibility tests for XTZ were carried out in terms of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), using broth microdilution method with endpoint after 48 h. Time-kill curves were determined at concentrations ranging from 0 to 25 μg ml-1. The MIC values of XTZ against M. furfur and M. pachydermatis were 1.25 and 0.25 μg ml-1, respectively. The MFC of XTZ was 5 μg ml-1 for M. furfur and 2.5 μg ml-1 for M. pachydermatis. Time-kill curves demonstrated that treatment with 25 μg ml-1 of XTZ for 5 h was able to kill 100{\%} of M. furfur, while 20 μg ml-1 of XTZ for 15 min killed M. pachydermatis completely. Conclusion: XTZ shows potential as an anti-Malassezia agent for inhibiting the growth of M. furfur ATCC 14521 and M. pachydermatis ATCC 14522 in vitro. Significance and Impact of the Study: XTZ may be a useful alternative for treating Malassezia-associated diseases.",
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In vitro anti-Malassezia activity of xanthorrhizol isolated from Curcuma xanthorrhiza Roxb. / Rukayadi, Y.; Hwang, J. K.

In: Letters in Applied Microbiology, Vol. 44, No. 2, 01.02.2007, p. 126-130.

Research output: Contribution to journalArticle

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