In vitro antibacterial and morphological effects of the urushiol component of the sap of the Korean lacquer tree (Rhus vernicifera stokes) on Helicobacter pylori

Ki Tae Suk, Hyun Soo Kim, Moon Young Kim, Jae Woo Kim, Young Uh, In Ho Jang, Soo Ki Kim, Eung Ho Choi, Myong Jo Kim, Jung Soo Joo, Soon Koo Baik

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Eradication regimens for Helicobacter pylori infection have some side effects, compliance problems, relapses, and antibiotic resistance. Therefore, alternative anti-H. pylori or supportive antimicrobial agents with fewer disadvantages are necessary for the treatment of H. pylori. We investigated the pH-(5.0, 6.0, 7.0, 8.0, 9.0, and 10.0) and concentration (0.032, 0.064, 0.128, 0.256, 0.514, and 1.024 mg/mL)-dependent antibacterial activity of crude urushiol extract from the sap of the Korean lacquer tree (Rhus vernicifera Stokes) against 3 strains (NCTC11637, 69, and 219) of H. pylori by the agar dilution method. In addition, the serial (before incubation, 3, 6, and 10 min after incubation) morphological effects of urushiol on H. pylori were examined by electron microscopy. All strains survived only within pH 6.0-9.0. The minimal inhibitory concentrations of the extract against strains ranged from 0.064 mg/mL to 0.256 mg/mL. Urushiol caused mainly separation of the membrane, vacuolization, and lysis of H. pylori. Interestingly, these changes were observed within 10 min following incubation with the 1×minimal inhibitory concentrations of urushiol. The results of this work suggest that urushiol has potential as a rapid therapeutic against H. pylori infection by disrupting the bacterial cell membrane.

Original languageEnglish
Pages (from-to)399-404
Number of pages6
JournalJournal of Korean medical science
Volume25
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

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Rhus
Lacquer
Helicobacter pylori
Helicobacter Infections
Microbial Drug Resistance
Anti-Infective Agents
urushiol
In Vitro Techniques
Complex Mixtures
Compliance
Agar
Electron Microscopy
Cell Membrane
Recurrence
Membranes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "In vitro antibacterial and morphological effects of the urushiol component of the sap of the Korean lacquer tree (Rhus vernicifera stokes) on Helicobacter pylori",
abstract = "Eradication regimens for Helicobacter pylori infection have some side effects, compliance problems, relapses, and antibiotic resistance. Therefore, alternative anti-H. pylori or supportive antimicrobial agents with fewer disadvantages are necessary for the treatment of H. pylori. We investigated the pH-(5.0, 6.0, 7.0, 8.0, 9.0, and 10.0) and concentration (0.032, 0.064, 0.128, 0.256, 0.514, and 1.024 mg/mL)-dependent antibacterial activity of crude urushiol extract from the sap of the Korean lacquer tree (Rhus vernicifera Stokes) against 3 strains (NCTC11637, 69, and 219) of H. pylori by the agar dilution method. In addition, the serial (before incubation, 3, 6, and 10 min after incubation) morphological effects of urushiol on H. pylori were examined by electron microscopy. All strains survived only within pH 6.0-9.0. The minimal inhibitory concentrations of the extract against strains ranged from 0.064 mg/mL to 0.256 mg/mL. Urushiol caused mainly separation of the membrane, vacuolization, and lysis of H. pylori. Interestingly, these changes were observed within 10 min following incubation with the 1×minimal inhibitory concentrations of urushiol. The results of this work suggest that urushiol has potential as a rapid therapeutic against H. pylori infection by disrupting the bacterial cell membrane.",
author = "Suk, {Ki Tae} and Kim, {Hyun Soo} and Kim, {Moon Young} and Kim, {Jae Woo} and Young Uh and Jang, {In Ho} and Kim, {Soo Ki} and Choi, {Eung Ho} and Kim, {Myong Jo} and Joo, {Jung Soo} and Baik, {Soon Koo}",
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In vitro antibacterial and morphological effects of the urushiol component of the sap of the Korean lacquer tree (Rhus vernicifera stokes) on Helicobacter pylori. / Suk, Ki Tae; Kim, Hyun Soo; Kim, Moon Young; Kim, Jae Woo; Uh, Young; Jang, In Ho; Kim, Soo Ki; Choi, Eung Ho; Kim, Myong Jo; Joo, Jung Soo; Baik, Soon Koo.

In: Journal of Korean medical science, Vol. 25, No. 3, 01.03.2010, p. 399-404.

Research output: Contribution to journalArticle

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AU - Suk, Ki Tae

AU - Kim, Hyun Soo

AU - Kim, Moon Young

AU - Kim, Jae Woo

AU - Uh, Young

AU - Jang, In Ho

AU - Kim, Soo Ki

AU - Choi, Eung Ho

AU - Kim, Myong Jo

AU - Joo, Jung Soo

AU - Baik, Soon Koo

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