Abstract
Recent discoveries of various forms of carbon nanostructure have stimulated research on their applications and hold promise for applications in medicine and many other related engineering areas. While carbon nanotubes (CNTs) are already being produced on a massive scale, few studies have been performed which test the potential harmful effects of this new technology. We used a 3-dimensional in vitro model of the human airway using a co-culture of normal human bronchial epithelial cells and normal human fibroblasts for the health risk assessment of CNTs on the human respiratory systems. We measured the production of nitric oxide (NO) as an inflammatory marker and MTT activity for cytotoxic response of the cell layers following exposure of different concentrations of CNTs. Our results indicated that NO production was dramatically increased and cell viability was decreased following exposure of different concentrations of CNTs. Further studies are required to get the transport properties of CNTs across the cells either in monolayer or in co-culture. In addition to inflammatory and cytotoxic responses of cell layers, the changes in physiological functions, such as mucin secretion, tight junction formation, and cilia formation, needs to be measured in the future, following exposure of different concentrations and structures of CNTs. This study will provide a science-based, comprehensive understanding of potential toxicity and ultimately enable the safer use of CNTs and CNT-based materials as novel nano-scale medical tools.
| Original language | English |
|---|---|
| Title of host publication | Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008 |
| Pages | 157-160 |
| Number of pages | 4 |
| Volume | 2 |
| Publication status | Published - 2008 Oct 1 |
| Event | 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 - Quebec City, QC, United States Duration: 2008 Jun 1 → 2008 Jun 5 |
Other
| Other | 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 |
|---|---|
| Country | United States |
| City | Quebec City, QC |
| Period | 08/6/1 → 08/6/5 |
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All Science Journal Classification (ASJC) codes
- Mechanical Engineering
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In Vitro diagnosis and analysis of the potential effects of carbon nanotube exposure on the human respiratory system. / Bowen, Emily; Purser, Forrest; Park, Young Bin; Lee, Joon Sang; Kwon, Soonjo.
Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. Vol. 2 2008. p. 157-160.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - In Vitro diagnosis and analysis of the potential effects of carbon nanotube exposure on the human respiratory system
AU - Bowen, Emily
AU - Purser, Forrest
AU - Park, Young Bin
AU - Lee, Joon Sang
AU - Kwon, Soonjo
PY - 2008/10/1
Y1 - 2008/10/1
N2 - Recent discoveries of various forms of carbon nanostructure have stimulated research on their applications and hold promise for applications in medicine and many other related engineering areas. While carbon nanotubes (CNTs) are already being produced on a massive scale, few studies have been performed which test the potential harmful effects of this new technology. We used a 3-dimensional in vitro model of the human airway using a co-culture of normal human bronchial epithelial cells and normal human fibroblasts for the health risk assessment of CNTs on the human respiratory systems. We measured the production of nitric oxide (NO) as an inflammatory marker and MTT activity for cytotoxic response of the cell layers following exposure of different concentrations of CNTs. Our results indicated that NO production was dramatically increased and cell viability was decreased following exposure of different concentrations of CNTs. Further studies are required to get the transport properties of CNTs across the cells either in monolayer or in co-culture. In addition to inflammatory and cytotoxic responses of cell layers, the changes in physiological functions, such as mucin secretion, tight junction formation, and cilia formation, needs to be measured in the future, following exposure of different concentrations and structures of CNTs. This study will provide a science-based, comprehensive understanding of potential toxicity and ultimately enable the safer use of CNTs and CNT-based materials as novel nano-scale medical tools.
AB - Recent discoveries of various forms of carbon nanostructure have stimulated research on their applications and hold promise for applications in medicine and many other related engineering areas. While carbon nanotubes (CNTs) are already being produced on a massive scale, few studies have been performed which test the potential harmful effects of this new technology. We used a 3-dimensional in vitro model of the human airway using a co-culture of normal human bronchial epithelial cells and normal human fibroblasts for the health risk assessment of CNTs on the human respiratory systems. We measured the production of nitric oxide (NO) as an inflammatory marker and MTT activity for cytotoxic response of the cell layers following exposure of different concentrations of CNTs. Our results indicated that NO production was dramatically increased and cell viability was decreased following exposure of different concentrations of CNTs. Further studies are required to get the transport properties of CNTs across the cells either in monolayer or in co-culture. In addition to inflammatory and cytotoxic responses of cell layers, the changes in physiological functions, such as mucin secretion, tight junction formation, and cilia formation, needs to be measured in the future, following exposure of different concentrations and structures of CNTs. This study will provide a science-based, comprehensive understanding of potential toxicity and ultimately enable the safer use of CNTs and CNT-based materials as novel nano-scale medical tools.
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M3 - Conference contribution
SN - 9781420085075
SN - 9781420085044
VL - 2
SP - 157
EP - 160
BT - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
ER -