In vitro mesenchymal stem cell culture using calcium phosphate glass scaffold

J. Kim, J. K. Ryu, M. C. Kim, Y. U. Kim, S. H. Choi, C. K. Kim, K. N. Kim, K. M. Kim, R. Z. LeGeros, Y. K. Lee

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to evaluate the cell affinity of calcium phosphate glass scaffold in the system of CaO-CaF2-P 2O5-MgO-ZnO, which is already reported that promoted the bone-like tissue formation in vitro and formed new bone in Sprague-Dawley rats. We prepared calcium phosphate glass saffolds with three-dimensionally interconnected pores of 200-500 μm. Commercial HA scaffold was employed as a control in this study. Bone marrow cells were collected from the healthy human donors and cultured within the prepared scaffolds. After 2, 4, 6, and 8 weeks, hMSCs/scaffold were fixed and stained with hematoxylin and eosin. hMSCs were continuously proliferated both in the experimental and control groups at every incubation period. The number of cells was higher in the experimental group than that of the control group, however, there was no significant difference (p>0.05). Extracellular matrices could be observed at the 2nd and 4th days in the experimental and control groups, respectively. The extracellular matrices were more abundant in the experimental group at all periods. The prepared calcium phosphate glass scaffolds are expected effective in bone tissue engineering.

Original languageEnglish
Pages (from-to)679-682
Number of pages4
JournalKey Engineering Materials
Volume284-286
Publication statusPublished - 2005 Dec 1

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Calcium phosphate
Scaffolds (biology)
Stem cells
Cell culture
Scaffolds
Bone
Glass
Hematoxylin
Eosine Yellowish-(YS)
Tissue engineering
Rats
Cells
Tissue
calcium phosphate

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Kim, J., Ryu, J. K., Kim, M. C., Kim, Y. U., Choi, S. H., Kim, C. K., ... Lee, Y. K. (2005). In vitro mesenchymal stem cell culture using calcium phosphate glass scaffold. Key Engineering Materials, 284-286, 679-682.
Kim, J. ; Ryu, J. K. ; Kim, M. C. ; Kim, Y. U. ; Choi, S. H. ; Kim, C. K. ; Kim, K. N. ; Kim, K. M. ; LeGeros, R. Z. ; Lee, Y. K. / In vitro mesenchymal stem cell culture using calcium phosphate glass scaffold. In: Key Engineering Materials. 2005 ; Vol. 284-286. pp. 679-682.
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Kim, J, Ryu, JK, Kim, MC, Kim, YU, Choi, SH, Kim, CK, Kim, KN, Kim, KM, LeGeros, RZ & Lee, YK 2005, 'In vitro mesenchymal stem cell culture using calcium phosphate glass scaffold', Key Engineering Materials, vol. 284-286, pp. 679-682.

In vitro mesenchymal stem cell culture using calcium phosphate glass scaffold. / Kim, J.; Ryu, J. K.; Kim, M. C.; Kim, Y. U.; Choi, S. H.; Kim, C. K.; Kim, K. N.; Kim, K. M.; LeGeros, R. Z.; Lee, Y. K.

In: Key Engineering Materials, Vol. 284-286, 01.12.2005, p. 679-682.

Research output: Contribution to journalArticle

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