In vitro red fluorescence as an indicator of caries lesion activity

Bo Ra Kim, Si Mook Kang, Elbert De Josselin De Jong, Ho Keun Kwon, Baek Il Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


This in vitro study examined the utility of comparing red fluorescence between active and inactive caries lesions and investigated whether changes in red fluorescence and fluorescence loss are influenced by lesion activity following remineralization. Sixty-two noncavitated smooth surface caries lesions on extracted human teeth were classified into active or inactive lesions using the Nyvad system prior to a 12-day pH-cycling procedure. Quantitative light-induced fluorescence-digital images were used to measure fluorescence parameters before and after pH cycling. At baseline, the intensity (DR) and area (ADR) of red fluorescence were 1.5- and 2.2-fold higher in active lesions than in inactive lesions (p,0.05). The ratio of ADR to lesion area was associated with classification of active lesions (odds ratio = 1.031; 95% confidence interval = 1.005-1.058). After pH cycling, the active lesions showed about 2- and 8-fold greater reductions in the median values of ADR and fluorescence loss related to lesion volume (DQ) compared with inactive lesions (p,0.05). In conclusion, red fluorescence differs depending on lesion activity, and the red fluorescence area and lesion volume change following remineralization. The results suggest that measuring red fluorescence may be a useful way of objectively evaluating lesion activity of smooth surface lesions.

Original languageEnglish
Pages (from-to)405-413
Number of pages9
JournalOperative dentistry
Issue number4
Publication statusPublished - 2019

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education under grant (2016R1D1A1A09916934).

Publisher Copyright:
© 2019 Indiana University School of Dentistry. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Dentistry(all)


Dive into the research topics of 'In vitro red fluorescence as an indicator of caries lesion activity'. Together they form a unique fingerprint.

Cite this