To study the effects of mesenchymal stem cells (MSCs) on cell regeneration and treatment, this method tracks MSC migration and morphological changes after coculture with cochlear epithelium. The organ of Corti was immobilized on a plastic coverslip by pressing a portion of the Reissner's membrane generated during the dissection. MSCs confined by a glass cylinder migrated toward cochlear epithelium when the cylinder was removed. Their predominant localization was observed in the modiolus of the organ of Corti, aligned in a direction similarly to that of the nerve fibers. However, some MSCs were localized in the limbus area and showed a horizontally elongated shape. In addition, migration into the hair cell area was increased, and the morphology of the MSCs changed to various forms after kanamycin treatment. In conclusion, the results of this study indicate that the coculture of MSCs with cochlear epithelium will be useful for the development of therapeutics via cell transplantation and for studies of cell regeneration that can examine various conditions and factors.
|Number of pages||12|
|Journal||Journal of Visualized Experiments|
|Publication status||Published - 2020 Dec|
Bibliographical noteFunding Information:
This work was supported by research grants (NRF-2018-R1D1A1B07050175, HURF-2017-66) from the National Research Foundation (NRF) of Korea and Hallym University Research Foundation.
This work was supported by research grants (NRF-2018 R1D1A1B07050175, HURF-2017-66) from the National Research Foundation (NRF) of Korea and Hallym University Research Foundation.
© 2020 JoVE Journal of Visualized Experiments.
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)