In vivo determination of the gap2 gene promoter activity in Giardia lamblia.

Hye Won Yang, Juri Kim, Tai Soon Yong, Soon Jung Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalThe Korean journal of parasitology
Volume44
Issue number1
DOIs
Publication statusPublished - 2006 Mar

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

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