In vivo effects of NbSiR silencing on chloroplast development in Nicotiana Benthamiana

Yong Won Kang, Jae Yong Lee, Young Jeon, Gang Won Cheong, Moonil Kim, Hyunsook Pai

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Sulfite reductase (SiR) performs dual functions, acting as a sulfur assimilation enzyme and as a chloroplast (cp-) nucleoid binding protein. In this study, we examined the in vivo effects of SiR deficiency on chloroplast development in Nicotiana benthamiana. Virus-induced gene silencing of NbSiR resulted in leaf yellowing and growth retardation phenotypes, which were not rescued by cysteine supplementation. NbSiR:GFP fusion protein was targeted to chloroplasts and colocalized with cp-nucleoids. Recombinant full-length NbSiR protein and the C-terminal half of NbSiR possessed cp-DNA compaction activities in vitro, and expression of full-length NbSiR in E. coli caused condensation of genomic DNA. NbSiR silencing differentially affected expression of plastid-encoded genes, inhibiting expression of several genes more severely than others. In the later stages, depletion of NbSiR resulted in chloroplast ablation. In NbSiR-silenced plants, enlarged cp-nucleoids containing an increased amount of cp-DNA were observed in the middle of the abnormal chloroplasts, and the cp-DNAs were predominantly of subgenomic sizes based on pulse field gel electrophoresis. The abnormal chloroplasts developed prolamellar body-like cubic lipid structures in the light without accumulating NADPH:protochlorophyllide oxidoreductase proteins. Our results suggest that NbSiR plays a role in cp-nucleoid metabolism, plastid gene expression, and thylakoid membrane development.

Original languageEnglish
Pages (from-to)569-583
Number of pages15
JournalPlant Molecular Biology
Volume72
Issue number6
DOIs
Publication statusPublished - 2010 Jan 4

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Nicotiana benthamiana
Chloroplasts
Tobacco
chloroplasts
Chloroplast DNA
Oxidoreductases Acting on Sulfur Group Donors
chloroplast DNA
Plastids
sulfites
protochlorophyllide reductase
plastids
protochlorophyllides
Gene Expression
gene expression
Thylakoids
proteins
oxidoreductases
Gene Silencing
gene silencing
Protein C

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Kang, Yong Won ; Lee, Jae Yong ; Jeon, Young ; Cheong, Gang Won ; Kim, Moonil ; Pai, Hyunsook. / In vivo effects of NbSiR silencing on chloroplast development in Nicotiana Benthamiana. In: Plant Molecular Biology. 2010 ; Vol. 72, No. 6. pp. 569-583.
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In vivo effects of NbSiR silencing on chloroplast development in Nicotiana Benthamiana. / Kang, Yong Won; Lee, Jae Yong; Jeon, Young; Cheong, Gang Won; Kim, Moonil; Pai, Hyunsook.

In: Plant Molecular Biology, Vol. 72, No. 6, 04.01.2010, p. 569-583.

Research output: Contribution to journalArticle

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