In vivo efficacy of paclitaxel-loaded injectable in situ-forming gel against subcutaneous tumor growth

Ju Young Lee, Kyung Sook Kim, Yun Mi Kang, E. Sle Kim, Sung Joo Hwang, Hai Bang Lee, Byoung Hyun Min, Jae Ho Kim, Moon Suk Kim

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Injectable in situ-forming gels have received considerable attention as localized drug delivery systems. Here, we examined a poly(ethylene glycol)-b-polycaprolactone (MPEG-PCL) diblock copolymer gel as an injectable drug depot for paclitaxel (Ptx). The copolymer solution remained liquid at room temperature and rapidly gelled in vivo at body temperature. In vitro experiments showed that Ptx was released from MPEG-PCL copolymer gels over the course of more than 14 days. Experiments employing intratumoral injection of saline (control), gel-only, Taxol, or Ptx-loaded gel into mice bearing B16F10 tumor xenografts showed that Ptx-loaded gel inhibited the growth of B16F10 tumors more effectively than did saline or gel alone. Further, intratumoral injection of Ptx-loaded gel was more efficacious in inhibiting the growth of B16F10 tumor over 10 days than was injection of Taxol. A histological analysis demonstrated an increase in necrotic tissue in tumors treated with Ptx-loaded gel. In conclusion, our data show that intratumoral injection of Ptx-loaded MPEG-PCL diblock copolymer yielded an in situ-forming gel that exhibited controlled Ptx release profile, and that was effective in treating localized solid tumors.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalInternational Journal of Pharmaceutics
Volume392
Issue number1-2
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Paclitaxel
Gels
Injections
Growth
Neoplasms
Ethylene Glycol
Drug Delivery Systems
Body Temperature
Heterografts
Temperature

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Lee, Ju Young ; Kim, Kyung Sook ; Kang, Yun Mi ; Kim, E. Sle ; Hwang, Sung Joo ; Lee, Hai Bang ; Min, Byoung Hyun ; Kim, Jae Ho ; Kim, Moon Suk. / In vivo efficacy of paclitaxel-loaded injectable in situ-forming gel against subcutaneous tumor growth. In: International Journal of Pharmaceutics. 2010 ; Vol. 392, No. 1-2. pp. 51-56.
@article{62cb5e9c386446cdb245030eba360548,
title = "In vivo efficacy of paclitaxel-loaded injectable in situ-forming gel against subcutaneous tumor growth",
abstract = "Injectable in situ-forming gels have received considerable attention as localized drug delivery systems. Here, we examined a poly(ethylene glycol)-b-polycaprolactone (MPEG-PCL) diblock copolymer gel as an injectable drug depot for paclitaxel (Ptx). The copolymer solution remained liquid at room temperature and rapidly gelled in vivo at body temperature. In vitro experiments showed that Ptx was released from MPEG-PCL copolymer gels over the course of more than 14 days. Experiments employing intratumoral injection of saline (control), gel-only, Taxol, or Ptx-loaded gel into mice bearing B16F10 tumor xenografts showed that Ptx-loaded gel inhibited the growth of B16F10 tumors more effectively than did saline or gel alone. Further, intratumoral injection of Ptx-loaded gel was more efficacious in inhibiting the growth of B16F10 tumor over 10 days than was injection of Taxol. A histological analysis demonstrated an increase in necrotic tissue in tumors treated with Ptx-loaded gel. In conclusion, our data show that intratumoral injection of Ptx-loaded MPEG-PCL diblock copolymer yielded an in situ-forming gel that exhibited controlled Ptx release profile, and that was effective in treating localized solid tumors.",
author = "Lee, {Ju Young} and Kim, {Kyung Sook} and Kang, {Yun Mi} and Kim, {E. Sle} and Hwang, {Sung Joo} and Lee, {Hai Bang} and Min, {Byoung Hyun} and Kim, {Jae Ho} and Kim, {Moon Suk}",
year = "2010",
month = "6",
day = "1",
doi = "10.1016/j.ijpharm.2010.03.033",
language = "English",
volume = "392",
pages = "51--56",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1-2",

}

In vivo efficacy of paclitaxel-loaded injectable in situ-forming gel against subcutaneous tumor growth. / Lee, Ju Young; Kim, Kyung Sook; Kang, Yun Mi; Kim, E. Sle; Hwang, Sung Joo; Lee, Hai Bang; Min, Byoung Hyun; Kim, Jae Ho; Kim, Moon Suk.

In: International Journal of Pharmaceutics, Vol. 392, No. 1-2, 01.06.2010, p. 51-56.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vivo efficacy of paclitaxel-loaded injectable in situ-forming gel against subcutaneous tumor growth

AU - Lee, Ju Young

AU - Kim, Kyung Sook

AU - Kang, Yun Mi

AU - Kim, E. Sle

AU - Hwang, Sung Joo

AU - Lee, Hai Bang

AU - Min, Byoung Hyun

AU - Kim, Jae Ho

AU - Kim, Moon Suk

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Injectable in situ-forming gels have received considerable attention as localized drug delivery systems. Here, we examined a poly(ethylene glycol)-b-polycaprolactone (MPEG-PCL) diblock copolymer gel as an injectable drug depot for paclitaxel (Ptx). The copolymer solution remained liquid at room temperature and rapidly gelled in vivo at body temperature. In vitro experiments showed that Ptx was released from MPEG-PCL copolymer gels over the course of more than 14 days. Experiments employing intratumoral injection of saline (control), gel-only, Taxol, or Ptx-loaded gel into mice bearing B16F10 tumor xenografts showed that Ptx-loaded gel inhibited the growth of B16F10 tumors more effectively than did saline or gel alone. Further, intratumoral injection of Ptx-loaded gel was more efficacious in inhibiting the growth of B16F10 tumor over 10 days than was injection of Taxol. A histological analysis demonstrated an increase in necrotic tissue in tumors treated with Ptx-loaded gel. In conclusion, our data show that intratumoral injection of Ptx-loaded MPEG-PCL diblock copolymer yielded an in situ-forming gel that exhibited controlled Ptx release profile, and that was effective in treating localized solid tumors.

AB - Injectable in situ-forming gels have received considerable attention as localized drug delivery systems. Here, we examined a poly(ethylene glycol)-b-polycaprolactone (MPEG-PCL) diblock copolymer gel as an injectable drug depot for paclitaxel (Ptx). The copolymer solution remained liquid at room temperature and rapidly gelled in vivo at body temperature. In vitro experiments showed that Ptx was released from MPEG-PCL copolymer gels over the course of more than 14 days. Experiments employing intratumoral injection of saline (control), gel-only, Taxol, or Ptx-loaded gel into mice bearing B16F10 tumor xenografts showed that Ptx-loaded gel inhibited the growth of B16F10 tumors more effectively than did saline or gel alone. Further, intratumoral injection of Ptx-loaded gel was more efficacious in inhibiting the growth of B16F10 tumor over 10 days than was injection of Taxol. A histological analysis demonstrated an increase in necrotic tissue in tumors treated with Ptx-loaded gel. In conclusion, our data show that intratumoral injection of Ptx-loaded MPEG-PCL diblock copolymer yielded an in situ-forming gel that exhibited controlled Ptx release profile, and that was effective in treating localized solid tumors.

UR - http://www.scopus.com/inward/record.url?scp=77952740817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952740817&partnerID=8YFLogxK

U2 - 10.1016/j.ijpharm.2010.03.033

DO - 10.1016/j.ijpharm.2010.03.033

M3 - Article

C2 - 20298770

AN - SCOPUS:77952740817

VL - 392

SP - 51

EP - 56

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -