In vivo high-throughput profiling of CRISPR-Cpf1 activity

Hui K. Kim, Myungjae Song, Jinu Lee, A. Vipin Menon, Soobin Jung, Young Mook Kang, Jae W. Choi, Euijeon Woo, Hyun C. Koh, Jin Wu Nam, Hyongbum Kim

Research output: Contribution to journalArticle

115 Citations (Scopus)


CRISPR from Prevotella and Francisella 1 (Cpf1) is an effector endonuclease of the class 2 CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) gene editing system. We developed a method for evaluating Cpf1 activity, based on target sequence composition in mammalian cells, in a high-throughput manner. A library of >11,000 target sequence and guide RNA pairs was delivered into human cells using lentiviral vectors. Subsequent delivery of Cpf1 into this cell library induced insertions and deletions (indels) at the integrated synthetic target sequences, which allowed en masse evaluation of Cpf1 activity by using deep sequencing. With this approach, we determined protospacer-adjacent motif sequences of two Cpf1 nucleases, one from Acidaminococcus sp. BV3L6 (hereafter referred to as AsCpf1) and the other from Lachnospiraceae bacterium ND2006 (hereafter referred to as LbCpf1). We also defined target-sequence-dependent activity profiles of AsCpf1, which enabled the development of a web tool that predicts the indel frequencies for given target sequences ( Both the Cpf1 characterization profile and the in vivo high-throughput evaluation method will greatly facilitate Cpf1-based genome editing.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalNature Methods
Issue number2
Publication statusPublished - 2017 Feb 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kim, H. K., Song, M., Lee, J., Menon, A. V., Jung, S., Kang, Y. M., Choi, J. W., Woo, E., Koh, H. C., Nam, J. W., & Kim, H. (2017). In vivo high-throughput profiling of CRISPR-Cpf1 activity. Nature Methods, 14(2), 153-159.