In vivo identification of bipotential adipocyte progenitors recruited by β3-adrenoceptor activation and high-fat feeding

Yun Hee Lee, Anelia P. Petkova, Emilio P. Mottillo, James G. Granneman

Research output: Contribution to journalArticle

367 Citations (Scopus)

Abstract

Nutritional and pharmacological stimuli can dramatically alter the cellular phenotypes in white adipose tissue (WAT). Utilizing genetic lineage tracing techniques, we demonstrate that brown adipocytes (BA) that are induced by β3-adrenergic receptor activation in abdominal WAT arise from the proliferation and differentiation of cells expressing platelet-derived growth factor receptor alpha (PDGFRα), CD34, and Sca-1 (PDGFRα + cells). PDGFRα + cells have a unique morphology in which extended processes contact multiple cells in the tissue microenvironment. Surprisingly, these cells also give rise to white adipocytes (WA) that can comprise up to 25% of total fat cells in abdominal fat pads following 8 weeks of high-fat feeding. Isolated PDGFRα + cells differentiated into both BA and WA in vitro and generated WA after transplantation in vivo. The identification of PDGFRα + cells as bipotential adipocyte progenitors will enable further investigation of mechanisms that promote therapeutic cellular remodeling in adult WAT.

Original languageEnglish
Pages (from-to)480-491
Number of pages12
JournalCell Metabolism
Volume15
Issue number4
DOIs
Publication statusPublished - 2012 Apr 4

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Platelet-Derived Growth Factor alpha Receptor
Adipocytes
Adrenergic Receptors
White Adipocytes
Fats
White Adipose Tissue
Brown Adipocytes
Abdominal Fat
Adipose Tissue
Cell Differentiation
Transplantation
Cell Proliferation
Pharmacology
Phenotype

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Lee, Yun Hee ; Petkova, Anelia P. ; Mottillo, Emilio P. ; Granneman, James G. / In vivo identification of bipotential adipocyte progenitors recruited by β3-adrenoceptor activation and high-fat feeding. In: Cell Metabolism. 2012 ; Vol. 15, No. 4. pp. 480-491.
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In vivo identification of bipotential adipocyte progenitors recruited by β3-adrenoceptor activation and high-fat feeding. / Lee, Yun Hee; Petkova, Anelia P.; Mottillo, Emilio P.; Granneman, James G.

In: Cell Metabolism, Vol. 15, No. 4, 04.04.2012, p. 480-491.

Research output: Contribution to journalArticle

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