In vivo induction of mucosal immune responses by intranasal administration of chitosan microspheres containing Bordetella bronchiseptica DNT

Mi Lan Kang, Sang Gyun Kang, Hu Lin Jiang, Seung Won Shin, Deog Yong Lee, Jeong Min Ahn, Nabin Rayamahji, In Kyu Park, Sung Jae Shin, Chong Su Cho, Han Sang Yoo

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

In vitro immune-stimulating activities of Bordetella bronchiseptica dermonecrotoxin (BBD)-loaded in chitosan microspheres (CMs) were reported with a mouse alveolar macrophage cell line (RAW264.7). Based on the report, in vivo activity of immune-induction was investigated by intranasal administration of the BBD-loaded CMs into mice. BBD was loaded into the CMs prepared by an ionic gelation process with tripolyphosphate. Mice were immunized by direct administration of the BBD-loaded CMs into the nasal cavity. After immunization of the mice, BBD-specific immune responses (IgG and IgA titers) were measured in sera, nasal wash, and saliva by ELISA. BBD-specific IgA titers in the nasal cavity were time- and dose-dependently increased by the administration. Similar phenomena were observed in the analysis of systemic IgA and IgG in sera. However, the antibody in saliva was undetectable by ELISA. These results suggested that direct vaccination via the nasal cavity was effective for targeting nasal-associated lymphoid tissues, and that CMs were an efficient adjuvant in nasal mucosal immunity for atrophic rhinitis vaccine.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume63
Issue number2
DOIs
Publication statusPublished - 2006 Jun 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Pharmaceutical Science

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