In vivo magnetic resonance microscopy of differentiation in Xenopus laevis embryos from the first cleavage onwards

Seung Cheol Lee, Daniel Mietchen, Jee Hyun Cho, Young Sook Kim, Cheolsu Kim, Kwan Soo Hong, Chulhyun Lee, Dongmin Kang, Weon Tae Lee, Chaejoon Cheong

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Differentiation inside a developing embryo can be observed by a variety of optical methods but hardly so in opaque organisms. Embryos of the frog Xenopus laevis - a popular model system - belong to the latter category and, for this reason, are predominantly being investigated by means of physical sectioning. Magnetic resonance imaging (MRI) is a noninvasive method independent of the optical opaqueness of the object. Starting out from clinical diagnostics, the technique has now developed into a branch of microscopy - MR microscopy - that provides spatial resolutions of tens of microns for small biological objects. Nondestructive three-dimensional images of various embryos have been obtained using this technique. They were, however, usually acquired by long scans of fixed embryos. Previously reported in vivo studies did not cover the very early embryonic stages, mainly for sensitivity reasons. Here, by applying high field MR microscopy to the X. laevis system, we achieved the temporal and spatial resolution required for observing subcellular dynamics during early cell divisions in vivo. We present image series of dividing cells and nuclei and of the whole embryonic development from the zygote onto the hatching of the tadpole. Additionally, biomechanical analyses from successive MR images are introduced. These results demonstrate that MR microscopy can provide unique contributions to investigations of differentiating cells and tissues in vivo.

Original languageEnglish
Pages (from-to)84-92
Number of pages9
JournalDifferentiation
Volume75
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Xenopus laevis
Microscopy
Magnetic Resonance Spectroscopy
Embryonic Structures
Three-Dimensional Imaging
Zygote
Cell Nucleus
Anura
Cell Division
Embryonic Development
Larva
Magnetic Resonance Imaging

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

Cite this

Lee, Seung Cheol ; Mietchen, Daniel ; Cho, Jee Hyun ; Kim, Young Sook ; Kim, Cheolsu ; Hong, Kwan Soo ; Lee, Chulhyun ; Kang, Dongmin ; Lee, Weon Tae ; Cheong, Chaejoon. / In vivo magnetic resonance microscopy of differentiation in Xenopus laevis embryos from the first cleavage onwards. In: Differentiation. 2007 ; Vol. 75, No. 1. pp. 84-92.
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Lee, SC, Mietchen, D, Cho, JH, Kim, YS, Kim, C, Hong, KS, Lee, C, Kang, D, Lee, WT & Cheong, C 2007, 'In vivo magnetic resonance microscopy of differentiation in Xenopus laevis embryos from the first cleavage onwards', Differentiation, vol. 75, no. 1, pp. 84-92. https://doi.org/10.1111/j.1432-0436.2006.00114.x

In vivo magnetic resonance microscopy of differentiation in Xenopus laevis embryos from the first cleavage onwards. / Lee, Seung Cheol; Mietchen, Daniel; Cho, Jee Hyun; Kim, Young Sook; Kim, Cheolsu; Hong, Kwan Soo; Lee, Chulhyun; Kang, Dongmin; Lee, Weon Tae; Cheong, Chaejoon.

In: Differentiation, Vol. 75, No. 1, 01.01.2007, p. 84-92.

Research output: Contribution to journalArticle

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