Abstract
Exposure to mercury causes severe damage to various tissues and organs in humans. Concern over mercury toxicity has encouraged the development of efficient, sensitive, and selective methods for the in vivo detection of mercury. Although a variety of chemosensors have been exploited for this purpose, no in vivo monitoring systems have been described to date. In this report, we describe an irreversible rhodamine chemosensor-based, real-time monitoring system to detect mercury ions in living cells and, in particular, vertebrate organisms. The chemosensor responds rapidly, irreversibly, and stoichiometrically to mercury ions in aqueous media at room temperature. The results of experiments with mammalian cells and zebrafish show that the mercury chemosensor is cell and organism permeable and that it responds selectively to mercury ions over other metal ions. In addition, real-time monitoring of mercury-ion uptake by cells and zebrafish using this chemosensor shows that saturation of mercury-ion uptake occurs within 20-30 min in cells and organisms. Finally, accumulation of mercury ions in zebrafish tissue and organs is readily detected by using this rhodamine-based chemosensor.
| Original language | English |
|---|---|
| Pages (from-to) | 14150-14155 |
| Number of pages | 6 |
| Journal | Journal of the American Chemical Society |
| Volume | 128 |
| Issue number | 43 |
| DOIs | |
| Publication status | Published - 2006 Nov 1 |
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All Science Journal Classification (ASJC) codes
- Catalysis
- Chemistry(all)
- Biochemistry
- Colloid and Surface Chemistry
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In vivo monitoring of mercury ions using a rhodamine-based molecular probe. / Ko, Sung Kyun; Yang, Young Keun; Tae, Jinsung; Shin, Injae.
In: Journal of the American Chemical Society, Vol. 128, No. 43, 01.11.2006, p. 14150-14155.Research output: Contribution to journal › Article
TY - JOUR
T1 - In vivo monitoring of mercury ions using a rhodamine-based molecular probe
AU - Ko, Sung Kyun
AU - Yang, Young Keun
AU - Tae, Jinsung
AU - Shin, Injae
PY - 2006/11/1
Y1 - 2006/11/1
N2 - Exposure to mercury causes severe damage to various tissues and organs in humans. Concern over mercury toxicity has encouraged the development of efficient, sensitive, and selective methods for the in vivo detection of mercury. Although a variety of chemosensors have been exploited for this purpose, no in vivo monitoring systems have been described to date. In this report, we describe an irreversible rhodamine chemosensor-based, real-time monitoring system to detect mercury ions in living cells and, in particular, vertebrate organisms. The chemosensor responds rapidly, irreversibly, and stoichiometrically to mercury ions in aqueous media at room temperature. The results of experiments with mammalian cells and zebrafish show that the mercury chemosensor is cell and organism permeable and that it responds selectively to mercury ions over other metal ions. In addition, real-time monitoring of mercury-ion uptake by cells and zebrafish using this chemosensor shows that saturation of mercury-ion uptake occurs within 20-30 min in cells and organisms. Finally, accumulation of mercury ions in zebrafish tissue and organs is readily detected by using this rhodamine-based chemosensor.
AB - Exposure to mercury causes severe damage to various tissues and organs in humans. Concern over mercury toxicity has encouraged the development of efficient, sensitive, and selective methods for the in vivo detection of mercury. Although a variety of chemosensors have been exploited for this purpose, no in vivo monitoring systems have been described to date. In this report, we describe an irreversible rhodamine chemosensor-based, real-time monitoring system to detect mercury ions in living cells and, in particular, vertebrate organisms. The chemosensor responds rapidly, irreversibly, and stoichiometrically to mercury ions in aqueous media at room temperature. The results of experiments with mammalian cells and zebrafish show that the mercury chemosensor is cell and organism permeable and that it responds selectively to mercury ions over other metal ions. In addition, real-time monitoring of mercury-ion uptake by cells and zebrafish using this chemosensor shows that saturation of mercury-ion uptake occurs within 20-30 min in cells and organisms. Finally, accumulation of mercury ions in zebrafish tissue and organs is readily detected by using this rhodamine-based chemosensor.
UR - http://www.scopus.com/inward/record.url?scp=33750458684&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750458684&partnerID=8YFLogxK
U2 - 10.1021/ja065114a
DO - 10.1021/ja065114a
M3 - Article
C2 - 17061899
AN - SCOPUS:33750458684
VL - 128
SP - 14150
EP - 14155
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 43
ER -