In vivo MR imaging of tissue-engineered human mesenchymal stem cells transplanted to mouse: A preliminary study

In Kap Ko, Ho Taek Song, Eun Jin Cho, Eun Sook Lee, Yong Min Huh, Jin Suck Suh

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Current progress integrating stem cell biology and tissue engineering techniques has been invaluable to clinical applications. Prior to the application of celluar transplantation technique to patients, we need to establish techniques that can monitor their tissue biodistribution non-invasively. In this study, we proposed an imaging modality using MRI to not only monitor implanted scaffold in vivo, but also to track transplanted cells and behavior around the implant. For this purpose, human bone marrow-derived mesenchymal stem cells (hMSCs) were labeled with superparamagnetic iron oxide (Feridex) and then labeled hMSCs were cultured in a gelatin sponge used as a scaffold to support cell growth and proliferation. Histological assessment and MTT assay showed that cell labeling with MR contrast agent did not harm cell viability. Also, Feridex-labeled hMSCs showed a significant decrease in T2 signal intensity, even within the gelatin sponge in vitro. After implanting the sponge/cell complex in vivo, we could visualize cellular behavior around the implant over time using a noninvasive MRI modality and this finding was correlated with histological study, which illustrates the potential of a new approach proposed here for in vivo monitoring of implanted cell-based tissue-engineered product.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalAnnals of Biomedical Engineering
Volume35
Issue number1
DOIs
Publication statusPublished - 2007 Jan

Bibliographical note

Funding Information:
We would like to thank to Prof. S.-H. Moon in Department of Orthopaedic Surgery in Yonsei University for his kind hMSC donation. This work was supported by the Korea Research Foundation Grant (KRF-2004-003-E00171).

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

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