In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe

Xiaoqiang Chen, Dayoung Lee, Sungsook Yu, Gyoungmi Kim, Songyi Lee, Yejin Cho, Haengdueng Jeong, KiTaek Nam, Juyoung Yoon

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe (CyA-P-CyB) and its applications as an NIR imaging probe for tumor cells and as a phototherapy reagent for tumors. In vitro experiments demonstrated that CyA-P-CyB was activated via the cleavage of a peptide linker by cathepsin B in tumor cells to produce fluorescence in the NIR region based on a FRET mechanism. MTT assays showed that the phototoxicity of CyA-P-CyB toward cells depended on the activity of cathepsin B, and the probe exhibited specific phototoxicity toward tumor cells. CyA-P-CyB was also successfully applied to the in vivo imaging and phototherapy of tumors. Histological analysis indicated that CyA-P-CyB had no cytotoxic effects on seven mouse tissues (lung, liver, heart, kidney, pancreas, spleen and brain) after the CyA-P-CyB treatment and laser irradiation.

Original languageEnglish
Pages (from-to)130-140
Number of pages11
JournalBiomaterials
Volume122
DOIs
Publication statusPublished - 2017 Apr 1

Fingerprint

Cathepsin B
Phototherapy
Infrared imaging
Fluorescent Dyes
Tumors
Phototoxic Dermatitis
Neoplasms
Cells
Laser beam effects
Liver
Peptides
Cathepsins
Pancreas
Assays
Brain
Lasers
Spleen
Fluorescence
Tissue
Infrared radiation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Chen, Xiaoqiang ; Lee, Dayoung ; Yu, Sungsook ; Kim, Gyoungmi ; Lee, Songyi ; Cho, Yejin ; Jeong, Haengdueng ; Nam, KiTaek ; Yoon, Juyoung. / In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe. In: Biomaterials. 2017 ; Vol. 122. pp. 130-140.
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In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe. / Chen, Xiaoqiang; Lee, Dayoung; Yu, Sungsook; Kim, Gyoungmi; Lee, Songyi; Cho, Yejin; Jeong, Haengdueng; Nam, KiTaek; Yoon, Juyoung.

In: Biomaterials, Vol. 122, 01.04.2017, p. 130-140.

Research output: Contribution to journalArticle

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