In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration

Jung U. Shin, Ji Yeon Noh, Ju Hee Lee, Won Jai Lee, Jong Shin Yoo, Jin Young Kim, Hyeran Kim, Inhee Jung, Shan Jin, Kwang Hoon Lee

Research output: Contribution to journalLetter

5 Citations (Scopus)

Abstract

It is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re-epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry was performed. Using SILAC, quantification of 1085 proteins was achieved. Among these proteins, 60 proteins were upregulated and 32 proteins were downregulated. Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) has been further evaluated for its role in the effect of oestrogen on keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to significantly accelerate the rate of HaCaT cell migration. To determine whether HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. Collectively, these experiments demonstrate that HMGB1 is a novel downstream mediator of oestrogen-stimulated keratinocyte migration.

Original languageEnglish
Pages (from-to)478-480
Number of pages3
JournalExperimental dermatology
Volume24
Issue number6
DOIs
Publication statusPublished - 2015 Jun 1

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High Mobility Group Proteins
Keratinocytes
Proteomics
Estrogens
Proteins
Cell Movement
Re-Epithelialization
Isotope Labeling
Cell culture
Isotopes
Wound Healing
Labeling
Small Interfering RNA
Extracellular Matrix
Mass spectrometry
Mass Spectrometry
Skin
Down-Regulation
Cell Culture Techniques
Cytokines

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Shin, Jung U. ; Noh, Ji Yeon ; Lee, Ju Hee ; Lee, Won Jai ; Yoo, Jong Shin ; Kim, Jin Young ; Kim, Hyeran ; Jung, Inhee ; Jin, Shan ; Lee, Kwang Hoon. / In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration. In: Experimental dermatology. 2015 ; Vol. 24, No. 6. pp. 478-480.
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In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration. / Shin, Jung U.; Noh, Ji Yeon; Lee, Ju Hee; Lee, Won Jai; Yoo, Jong Shin; Kim, Jin Young; Kim, Hyeran; Jung, Inhee; Jin, Shan; Lee, Kwang Hoon.

In: Experimental dermatology, Vol. 24, No. 6, 01.06.2015, p. 478-480.

Research output: Contribution to journalLetter

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AU - Noh, Ji Yeon

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AU - Lee, Won Jai

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AU - Kim, Jin Young

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AU - Jung, Inhee

AU - Jin, Shan

AU - Lee, Kwang Hoon

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N2 - It is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re-epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry was performed. Using SILAC, quantification of 1085 proteins was achieved. Among these proteins, 60 proteins were upregulated and 32 proteins were downregulated. Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) has been further evaluated for its role in the effect of oestrogen on keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to significantly accelerate the rate of HaCaT cell migration. To determine whether HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. Collectively, these experiments demonstrate that HMGB1 is a novel downstream mediator of oestrogen-stimulated keratinocyte migration.

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