In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration

Jung U. Shin, Ji Yeon Noh, JuHee Lee, Won Jai Lee, Jong Shin Yoo, Jin Young Kim, Hyeran Kim, Inhee Jung, Shan Jin, Kwanghoon Lee

Research output: Contribution to journalLetter

4 Citations (Scopus)

Abstract

It is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re-epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry was performed. Using SILAC, quantification of 1085 proteins was achieved. Among these proteins, 60 proteins were upregulated and 32 proteins were downregulated. Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) has been further evaluated for its role in the effect of oestrogen on keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to significantly accelerate the rate of HaCaT cell migration. To determine whether HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. Collectively, these experiments demonstrate that HMGB1 is a novel downstream mediator of oestrogen-stimulated keratinocyte migration.

Original languageEnglish
Pages (from-to)478-480
Number of pages3
JournalExperimental dermatology
Volume24
Issue number6
DOIs
Publication statusPublished - 2015 Jun 1

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High Mobility Group Proteins
Keratinocytes
Proteomics
Estrogens
Proteins
Cell Movement
Re-Epithelialization
Isotope Labeling
Cell culture
Isotopes
Wound Healing
Labeling
Small Interfering RNA
Extracellular Matrix
Mass spectrometry
Mass Spectrometry
Skin
Down-Regulation
Cell Culture Techniques
Cytokines

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Shin, Jung U. ; Noh, Ji Yeon ; Lee, JuHee ; Lee, Won Jai ; Yoo, Jong Shin ; Kim, Jin Young ; Kim, Hyeran ; Jung, Inhee ; Jin, Shan ; Lee, Kwanghoon. / In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration. In: Experimental dermatology. 2015 ; Vol. 24, No. 6. pp. 478-480.
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In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration. / Shin, Jung U.; Noh, Ji Yeon; Lee, JuHee; Lee, Won Jai; Yoo, Jong Shin; Kim, Jin Young; Kim, Hyeran; Jung, Inhee; Jin, Shan; Lee, Kwanghoon.

In: Experimental dermatology, Vol. 24, No. 6, 01.06.2015, p. 478-480.

Research output: Contribution to journalLetter

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AU - Shin, Jung U.

AU - Noh, Ji Yeon

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AU - Lee, Won Jai

AU - Yoo, Jong Shin

AU - Kim, Jin Young

AU - Kim, Hyeran

AU - Jung, Inhee

AU - Jin, Shan

AU - Lee, Kwanghoon

PY - 2015/6/1

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N2 - It is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re-epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry was performed. Using SILAC, quantification of 1085 proteins was achieved. Among these proteins, 60 proteins were upregulated and 32 proteins were downregulated. Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) has been further evaluated for its role in the effect of oestrogen on keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to significantly accelerate the rate of HaCaT cell migration. To determine whether HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. Collectively, these experiments demonstrate that HMGB1 is a novel downstream mediator of oestrogen-stimulated keratinocyte migration.

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