In vivo requirement of activator-specific binding targets of Mediator

J. M. Park, H. S. Kim, S. J. Han, M. S. Hwang, Y. C. Lee, Y. J. Kim

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

There has been no unequivocal demonstration that the activator binding targets identified in vitro play a key role in transcriptional activation in viva. To examine whether activator-Mediator interactions are required for gene transcription under physiological conditions, we performed functional analyses with Mediator components that interact specifically with natural yeast activators. Different activators interact with Mediator via distinct binding targets. Deletion of a distinct activator binding region of Mediator completely compromised gene activation in viva by some, but not all, transcriptional activators. These demonstrate that the activator-specific targets in Mediator are essential for transcriptional activation in living cells, but their requirement was affected by the nature of the activator-DNA interaction and the existence of a postrecruitment activation process.

Original languageEnglish
Pages (from-to)8709-8719
Number of pages11
JournalMolecular and cellular biology
Volume20
Issue number23
DOIs
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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