Biomedical in vivo sensing methods in the near-infrared (NIR) range, which that provide relatively high photon transparency, separation from auto-fluorescence background, and extended sensitivity, are being used increasingly for non-invasive mapping and monitoring of molecular events in cancer cells. In this study, we fabricated an NIR fluorogenic nanosensor based on the nanoparticle surface energy transfer effect, by conjugation of fluorescent proteolytic enzyme-specific cleavable peptides with gold nanorods (GNRs). Membrane-anchored membrane type 1-matrix metalloproteinases (MT1-MMPs), a family of zinc-dependent proteolytic enzymes, can induce the metastatic potential of cancer cells by promoting degradation of the extracellular matrix. Therefore, sensitive detection of MT1-MMP activity can provide essential information in the clinical setting. We have applied in vivo NIR sensing to evaluate MT1-MMP activity, as an NIR imaging target, in an MT1-MMP-expressing metastatic tumor mouse model.
Bibliographical noteFunding Information:
This work was supported by a National Research Foundation of Korea (NRF) grant, funded by the Korean government, Ministry of Education and Science Technology (MEST) ( 2012R1A1A2006248 and 2014R1A1A2059806 ) and a grant from the National RD Program for Cancer Control, Ministry of Health and Welfare, Republic of Korea ( 1220100 ).
All Science Journal Classification (ASJC) codes
- Biomedical Engineering