In vivo sensing of proteolytic activity with an NSET-based NIR fluorogenic nanosensor

Minhee Ku, Yoochan Hong, Dan Heo, Eugene Lee, Seungyeon Hwang, Jin Suck Suh, Jaemoon Yang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Biomedical in vivo sensing methods in the near-infrared (NIR) range, which that provide relatively high photon transparency, separation from auto-fluorescence background, and extended sensitivity, are being used increasingly for non-invasive mapping and monitoring of molecular events in cancer cells. In this study, we fabricated an NIR fluorogenic nanosensor based on the nanoparticle surface energy transfer effect, by conjugation of fluorescent proteolytic enzyme-specific cleavable peptides with gold nanorods (GNRs). Membrane-anchored membrane type 1-matrix metalloproteinases (MT1-MMPs), a family of zinc-dependent proteolytic enzymes, can induce the metastatic potential of cancer cells by promoting degradation of the extracellular matrix. Therefore, sensitive detection of MT1-MMP activity can provide essential information in the clinical setting. We have applied in vivo NIR sensing to evaluate MT1-MMP activity, as an NIR imaging target, in an MT1-MMP-expressing metastatic tumor mouse model.

Original languageEnglish
Pages (from-to)471-477
Number of pages7
JournalBiosensors and Bioelectronics
Volume77
DOIs
Publication statusPublished - 2016 Mar 15

Fingerprint

Nanosensors
Matrix Metalloproteinase 14
Infrared radiation
Membranes
Peptide Hydrolases
Cells
Nanotubes
Neoplasms
Energy Transfer
Infrared imaging
Nanorods
Photons
Interfacial energy
Gold
Nanoparticles
Energy transfer
Transparency
Extracellular Matrix
Zinc
Tumors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Ku, Minhee ; Hong, Yoochan ; Heo, Dan ; Lee, Eugene ; Hwang, Seungyeon ; Suh, Jin Suck ; Yang, Jaemoon. / In vivo sensing of proteolytic activity with an NSET-based NIR fluorogenic nanosensor. In: Biosensors and Bioelectronics. 2016 ; Vol. 77. pp. 471-477.
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In vivo sensing of proteolytic activity with an NSET-based NIR fluorogenic nanosensor. / Ku, Minhee; Hong, Yoochan; Heo, Dan; Lee, Eugene; Hwang, Seungyeon; Suh, Jin Suck; Yang, Jaemoon.

In: Biosensors and Bioelectronics, Vol. 77, 15.03.2016, p. 471-477.

Research output: Contribution to journalArticle

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