In vivo tracking of human neural stem cells following transplantation into a rodent model of ischemic stroke

Da Jeong Chang, Hyeyoung Moon, Yong Hyun Lee, Nayeon Lee, Hong J. Lee, Iksoo Jeon, Hyunseung Lee, Tae Sun Hwang, Seung Hun Oh, Dong Ah Shin, Seung U. Kim, Kwan Soo Hong, Jihwan Song

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5 Citations (Scopus)

Abstract

Background and Objectives: Ischemic stroke caused by middle cerebral artery occlusion (MCAo) is the major type of stroke, but there are currently very limited options for cure. It has been shown that neural stem cells (NSCs) or neural precursor cells (NPCs) can survive and improve neurological deficits when they are engrafted in animal models of various neurological diseases. However, how the transplanted NSCs or NPCs are act in vivo in the injured or diseased brain is largely unknown. In this study, we utilized magnetic resonance imaging (MRI) techniques in order to understand the fates of human NSCs (HB1.F3) following transplantation into a rodent model of MCAo. Methods and Results: HB1.F3 human NSCs were pre-labeled with ferumoxides (Feridex®)-protamine sulfate complexes, which were visualized and examined by MRI up to 9 weeks after transplantation. Migration of the transplanted cells to the infarct area was further confirmed by histological methods. Conclusions: Based on these observations, we speculate that the transplanted NSCs have the extensive migratory ability to the injured site, which will in turn contribute to functional recovery in stroke.

Original languageEnglish
Pages (from-to)79-83
Number of pages5
JournalInternational Journal of Stem Cells
Volume5
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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    Chang, D. J., Moon, H., Lee, Y. H., Lee, N., Lee, H. J., Jeon, I., Lee, H., Hwang, T. S., Oh, S. H., Shin, D. A., Kim, S. U., Hong, K. S., & Song, J. (2012). In vivo tracking of human neural stem cells following transplantation into a rodent model of ischemic stroke. International Journal of Stem Cells, 5(1), 79-83. https://doi.org/10.15283/ijsc.2012.5.1.79