Inactivation of Mxi1 induces Il-8 secretion activation in polycystic kidney

Kyung Hyun Yoo, Young Hoon Sung, Moon Hee Yang, Jeong Ok Jeon, Yeon Joo Yook, Yu Mi Woo, Han Woong Lee, Jong Hoon Park

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13 Citations (Scopus)

Abstract

The Mxi1 proteins are biochemical and biological antagonists of c-myc oncoprotein. It has been reported that the overexpression pattern of c-myc might be similar to a molecular feature of early and late stages of human autosomal dominant polycystic kidney disease. We identified the cyst phenotype in Mxi1-deficient mice aged 6-12 months using H&E staining. Some chemokines containing a protein domain similar to human IL-8, which is associated with the inflammatory response, were subsequently selected from the up-regulated genes. We confirmed the expression level of these chemokines and measured protein concentrations of IL-8 using ELISA in the Mxi1-knockdown cells. IL-8 was found to be significantly increased in Mxi1-knockdown cells. We found that p38 MAP kinase activation was involved in the signal transduction of the Mxi1-inactivated secretion of IL-8. Therefore, we could suggest that the inactivation of Mxi1 leads to the inflammatory response and has the potential to induce polycystic renal disease.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number1
DOIs
Publication statusPublished - 2007 Apr 27

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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