Inactivation of Mxi1 induces Il-8 secretion activation in polycystic kidney

Kyung Hyun Yoo, Young Hoon Sung, Moon Hee Yang, Jeong Ok Jeon, Yeon Joo Yook, Yu Mi Woo, Han Woong Lee, Jong Hoon Park

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The Mxi1 proteins are biochemical and biological antagonists of c-myc oncoprotein. It has been reported that the overexpression pattern of c-myc might be similar to a molecular feature of early and late stages of human autosomal dominant polycystic kidney disease. We identified the cyst phenotype in Mxi1-deficient mice aged 6-12 months using H&E staining. Some chemokines containing a protein domain similar to human IL-8, which is associated with the inflammatory response, were subsequently selected from the up-regulated genes. We confirmed the expression level of these chemokines and measured protein concentrations of IL-8 using ELISA in the Mxi1-knockdown cells. IL-8 was found to be significantly increased in Mxi1-knockdown cells. We found that p38 MAP kinase activation was involved in the signal transduction of the Mxi1-inactivated secretion of IL-8. Therefore, we could suggest that the inactivation of Mxi1 leads to the inflammatory response and has the potential to induce polycystic renal disease.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number1
DOIs
Publication statusPublished - 2007 Apr 27

Fingerprint

Polycystic Kidney Diseases
Interleukin-8
Chemical activation
Chemokines
Autosomal Dominant Polycystic Kidney
Signal transduction
Proteins
Oncogene Proteins
p38 Mitogen-Activated Protein Kinases
Cysts
Signal Transduction
Genes
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Phenotype

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yoo, K. H., Sung, Y. H., Yang, M. H., Jeon, J. O., Yook, Y. J., Woo, Y. M., ... Park, J. H. (2007). Inactivation of Mxi1 induces Il-8 secretion activation in polycystic kidney. Biochemical and Biophysical Research Communications, 356(1), 85-90. https://doi.org/10.1016/j.bbrc.2007.02.103
Yoo, Kyung Hyun ; Sung, Young Hoon ; Yang, Moon Hee ; Jeon, Jeong Ok ; Yook, Yeon Joo ; Woo, Yu Mi ; Lee, Han Woong ; Park, Jong Hoon. / Inactivation of Mxi1 induces Il-8 secretion activation in polycystic kidney. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 356, No. 1. pp. 85-90.
@article{2bacffe0916a4a72b871cad83f8494b9,
title = "Inactivation of Mxi1 induces Il-8 secretion activation in polycystic kidney",
abstract = "The Mxi1 proteins are biochemical and biological antagonists of c-myc oncoprotein. It has been reported that the overexpression pattern of c-myc might be similar to a molecular feature of early and late stages of human autosomal dominant polycystic kidney disease. We identified the cyst phenotype in Mxi1-deficient mice aged 6-12 months using H&E staining. Some chemokines containing a protein domain similar to human IL-8, which is associated with the inflammatory response, were subsequently selected from the up-regulated genes. We confirmed the expression level of these chemokines and measured protein concentrations of IL-8 using ELISA in the Mxi1-knockdown cells. IL-8 was found to be significantly increased in Mxi1-knockdown cells. We found that p38 MAP kinase activation was involved in the signal transduction of the Mxi1-inactivated secretion of IL-8. Therefore, we could suggest that the inactivation of Mxi1 leads to the inflammatory response and has the potential to induce polycystic renal disease.",
author = "Yoo, {Kyung Hyun} and Sung, {Young Hoon} and Yang, {Moon Hee} and Jeon, {Jeong Ok} and Yook, {Yeon Joo} and Woo, {Yu Mi} and Lee, {Han Woong} and Park, {Jong Hoon}",
year = "2007",
month = "4",
day = "27",
doi = "10.1016/j.bbrc.2007.02.103",
language = "English",
volume = "356",
pages = "85--90",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

Inactivation of Mxi1 induces Il-8 secretion activation in polycystic kidney. / Yoo, Kyung Hyun; Sung, Young Hoon; Yang, Moon Hee; Jeon, Jeong Ok; Yook, Yeon Joo; Woo, Yu Mi; Lee, Han Woong; Park, Jong Hoon.

In: Biochemical and Biophysical Research Communications, Vol. 356, No. 1, 27.04.2007, p. 85-90.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inactivation of Mxi1 induces Il-8 secretion activation in polycystic kidney

AU - Yoo, Kyung Hyun

AU - Sung, Young Hoon

AU - Yang, Moon Hee

AU - Jeon, Jeong Ok

AU - Yook, Yeon Joo

AU - Woo, Yu Mi

AU - Lee, Han Woong

AU - Park, Jong Hoon

PY - 2007/4/27

Y1 - 2007/4/27

N2 - The Mxi1 proteins are biochemical and biological antagonists of c-myc oncoprotein. It has been reported that the overexpression pattern of c-myc might be similar to a molecular feature of early and late stages of human autosomal dominant polycystic kidney disease. We identified the cyst phenotype in Mxi1-deficient mice aged 6-12 months using H&E staining. Some chemokines containing a protein domain similar to human IL-8, which is associated with the inflammatory response, were subsequently selected from the up-regulated genes. We confirmed the expression level of these chemokines and measured protein concentrations of IL-8 using ELISA in the Mxi1-knockdown cells. IL-8 was found to be significantly increased in Mxi1-knockdown cells. We found that p38 MAP kinase activation was involved in the signal transduction of the Mxi1-inactivated secretion of IL-8. Therefore, we could suggest that the inactivation of Mxi1 leads to the inflammatory response and has the potential to induce polycystic renal disease.

AB - The Mxi1 proteins are biochemical and biological antagonists of c-myc oncoprotein. It has been reported that the overexpression pattern of c-myc might be similar to a molecular feature of early and late stages of human autosomal dominant polycystic kidney disease. We identified the cyst phenotype in Mxi1-deficient mice aged 6-12 months using H&E staining. Some chemokines containing a protein domain similar to human IL-8, which is associated with the inflammatory response, were subsequently selected from the up-regulated genes. We confirmed the expression level of these chemokines and measured protein concentrations of IL-8 using ELISA in the Mxi1-knockdown cells. IL-8 was found to be significantly increased in Mxi1-knockdown cells. We found that p38 MAP kinase activation was involved in the signal transduction of the Mxi1-inactivated secretion of IL-8. Therefore, we could suggest that the inactivation of Mxi1 leads to the inflammatory response and has the potential to induce polycystic renal disease.

UR - http://www.scopus.com/inward/record.url?scp=33947190405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947190405&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.02.103

DO - 10.1016/j.bbrc.2007.02.103

M3 - Article

C2 - 17350592

AN - SCOPUS:33947190405

VL - 356

SP - 85

EP - 90

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -